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. 1972 Dec;50(6):733–737. doi: 10.1104/pp.50.6.733

Detergent Extraction of Enzymes from Tobacco Leaves Varying in Maturity 1

Donald W De Jong a
PMCID: PMC366226  PMID: 16658253

Abstract

Enzyme activities of tobacco leaves were compared in detergent extracts. Highest levels of chlorogenic acid oxidase, malate-NAD oxidoreductase, and acid phosphatase were obtained from bud tissues. Peroxidase activity was least in young leaves and highest in senescent leaves yellowed with Ethrel. Peroxidase zymograms obtained by means of disc electrophoresis showed differences in isozyme composition among all five samples examined. Although protease was found in material extracted from buds, upper, middle, and lower leaf positions, none could be demonstrated in Ethrel-treated mature leaves.

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Selected References

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