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. 1972 Dec;50(6):774–777. doi: 10.1104/pp.50.6.774

pH Dependence and Cofactor Requirements of Photochemical Reactions in Maize Chloroplasts

D G Bishop a, Kirsten S Andersen a,1, Robert M Smillie a
PMCID: PMC366235  PMID: 16658262

Abstract

The pH dependence of the photoreduction of ferricyanide and the photoreduction of NADP from water and photosystem I activity have been compared in isolated chloroplasts from mesophyll and bundle sheath cells of Zea mays. The maximum activity of photoreduction of ferricyanide occurs at pH 8.5 in isolated mesophyll chloroplasts. The addition of methylamine does not cause a marked shift in the pH maximum, but brief sonication lowers the pH maximum to 7.0. In contrast, isolated bundle sheath chloroplasts have a pH maximum at 7.0 and the shape of the pH versus activity curve is similar to that of sonicated mesophyll chloroplasts. When photoreduction of ferricyanide by the isolated chloroplasts is measured at their pH maxima, the values for bundle sheath chloroplasts are about half those of methylamine-treated mesophyll chloroplasts on a chlorophyll basis.

The pH maxima for the photoreduction of NADP from water and photosystem I activity are similar in both mesophyll and bundle sheath chloroplasts with maximum activity occurring at pH 7.0 in both cases. In the presence of added plastocyanin and ferredoxin NADP-reductase, the photosystem I activities of both sonicated mesophyll and sonicated bundle sheath chloroplasts are significantly higher than those of the unsonicated preparations. On a chlorophyll basis, photosystem I activity of bundle sheath chloroplasts is at least twice that of mesophyll chloroplasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen K. S., Bain J. M., Bishop D. G., Smillie R. M. Photosystem II Activity in Agranal Bundle Sheath Chloroplasts from Zea mays. Plant Physiol. 1972 Apr;49(4):461–466. doi: 10.1104/pp.49.4.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. M., Boardman N. K., Spencer D. Phosphorylation by intact bundle sheath chloroplasts from maize. Biochim Biophys Acta. 1971 Aug 6;245(1):253–258. doi: 10.1016/0005-2728(71)90032-6. [DOI] [PubMed] [Google Scholar]
  3. Anderson J. M., Woo K. C., Boardman N. K. Photochemical systems in mesophyll and bundle sheath chloroplasts of C 4 plants. Biochim Biophys Acta. 1971 Sep 7;245(2):398–408. doi: 10.1016/0005-2728(71)90158-7. [DOI] [PubMed] [Google Scholar]
  4. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blackmon W. J., Moreland D. E. Adenosine triphosphatase activity associated with mung bean mitochondria. Plant Physiol. 1971 Apr;47(4):532–536. doi: 10.1104/pp.47.4.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Borchert M. T., Wessels J. S. Combined preparation of ferredoxin, ferredoxin-NADP+ reductase and plastocyanin from spinach leaves. Biochim Biophys Acta. 1970 Jan 13;197(1):78–83. doi: 10.1016/0005-2728(70)90010-1. [DOI] [PubMed] [Google Scholar]
  7. Forti G., Sturani E. On the structure and function of reduced nicotinamide adenine dinucleotide phosphate-cytochrome f reductase of spinach chloroplasts. Eur J Biochem. 1968 Feb;3(4):461–472. doi: 10.1111/j.1432-1033.1967.tb19553.x. [DOI] [PubMed] [Google Scholar]
  8. Mayne B. C. Spectral, Physical, and Electron Transport Activities in the Photosynthetic Apparatus of Mesophyll Cells and Bundle Sheath Cells of Digitaria sanguinalis (L.) Scop. Plant Physiol. 1971 May;47(5):600–605. doi: 10.1104/pp.47.5.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Neumann J., Jagendorf A. Uncoupling photophosphorylation by detergents. Biochim Biophys Acta. 1965 Nov 29;109(2):382–389. doi: 10.1016/0926-6585(65)90165-2. [DOI] [PubMed] [Google Scholar]
  10. O'neal D., Hew C. S., Latzko E., Gibbs M. Photosynthetic carbon metabolism of isolated corn chloroplasts. Plant Physiol. 1972 Apr;49(4):607–614. doi: 10.1104/pp.49.4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Polya G. M., Osmond C. B. Photophosphorylation by mesophyll and bundle sheath chloroplasts of c(4) plants. Plant Physiol. 1972 Feb;49(2):267–269. doi: 10.1104/pp.49.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STILLER M. FURTHER STUDIES ON PHOTOPHOSPHORYLATION ACCOMPANYING THE HILL REACTION WITH FERRICYANIDE. Biochim Biophys Acta. 1965 Jan 25;94:53–63. doi: 10.1016/0926-6585(65)90007-5. [DOI] [PubMed] [Google Scholar]
  13. Smillie R. M., Andersen K. S., Tobin N. F., Entsch B., Bishop D. G. Nicotinamide adenine dinucleotide phosphate photoreduction from water by agranal chloroplasts isolated from bundle sheath cells of maize. Plant Physiol. 1972 Apr;49(4):471–475. doi: 10.1104/pp.49.4.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Woo K. C., Anderson J. M., Boardman N. K., Downton W. J., Osmond C. B., Thorne S. W. Deficient Photosystem II in Agranal Bundle Sheath Chloroplasts of C(4) Plants. Proc Natl Acad Sci U S A. 1970 Sep;67(1):18–25. doi: 10.1073/pnas.67.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]

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