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. 1973 Feb;51(2):332–336. doi: 10.1104/pp.51.2.332

In Vivo Assay of Nitrate Reductase in Cotton Leaf Discs

Effect of Oxygen and Ammonium 1

J W Radin a
PMCID: PMC366260  PMID: 16658325

Abstract

Factors affecting nitrate reduction by leaf discs of cotton (Gossypium hirsutum L.) were investigated. When incubated in 30 mm nitrate, discs reduced nitrate much more slowly under air or O2 than under N2. Inhibition by O2 did not occur at nitrate levels of 100 mm or greater. Treatment with arsenate had little effect under N2 but stimulated nitrate reduction under air. Similarly, ammonium inhibited nitrate reduction, with the inhibition being partially relieved by arsenate. Uptake of nitrate was unaffected by ammonium. The NAD/NADH ratio increased in response to both oxygen and ammonium. The effects of these treatments on nitrate reduction can be explained by competition with nitrate for NADH generated by glycolysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Hayyim G., Gromet-Elhanan Z., Avron M. A specific and sensitive method for the determination of NADPH. Anal Biochem. 1969 Apr 4;28(1):6–12. doi: 10.1016/0003-2697(69)90151-1. [DOI] [PubMed] [Google Scholar]
  2. Ferrari T. E., Varner J. E. Control of nitrate reductase activity in barley aleurone layers. Proc Natl Acad Sci U S A. 1970 Mar;65(3):729–736. doi: 10.1073/pnas.65.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ferrari T. E., Varner J. E. Intact tissue assay for nitrite reductase in barley aleurone layers. Plant Physiol. 1971 Jun;47(6):790–794. doi: 10.1104/pp.47.6.790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fisher J. D., Hansen D., Hodges T. K. Correlation between ion fluxes and ion-stimulated adenosine triphosphatase activity of plant roots. Plant Physiol. 1970 Dec;46(6):812–814. doi: 10.1104/pp.46.6.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harper J. E. Canopy and Seasonal Profiles of Nitrate Reductase in Soybeans (Glycine max L. Merr.). Plant Physiol. 1972 Feb;49(2):146–154. doi: 10.1104/pp.49.2.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jaworski E. G. Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun. 1971 Jun 18;43(6):1274–1279. doi: 10.1016/s0006-291x(71)80010-4. [DOI] [PubMed] [Google Scholar]
  7. Kende H., Hahn H., Kays S. E. Enhancement of Nitrate Reductase Activity by Benzyladenine in Agrostemma githago. Plant Physiol. 1971 Dec;48(6):702–706. doi: 10.1104/pp.48.6.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klepper L., Flesher D., Hageman R. H. Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Plant Physiol. 1971 Nov;48(5):580–590. doi: 10.1104/pp.48.5.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leech R. M., Kirk P. R. An NADP-dependent L-glutamate dehydrogenase from chloroplasts of Vicia faba L. Biochem Biophys Res Commun. 1968 Aug 21;32(4):685–690. doi: 10.1016/0006-291x(68)90293-3. [DOI] [PubMed] [Google Scholar]
  10. Pahlich E., Joy K. W. Glutamate dehydrogenase from pea roots: purification and properties of the enzyme. Can J Biochem. 1971 Jan;49(1):127–138. doi: 10.1139/o71-018. [DOI] [PubMed] [Google Scholar]
  11. Streeter J. G., Bosler M. E. Comparison of in vitro and in vivo assays for nitrate reductase in soybean leaves. Plant Physiol. 1972 Mar;49(3):448–450. doi: 10.1104/pp.49.3.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yamamoto Y. Pyridine Nucleotide Content in the Higher Plant. Effect of Age of Tissue. Plant Physiol. 1963 Jan;38(1):45–54. doi: 10.1104/pp.38.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]

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