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. 1973 Feb;51(2):357–362. doi: 10.1104/pp.51.2.357

The Stoichiometry of Respiration-driven Potassium Transport in Corn Mitochondria 1

Betty I Kirk a,2,3, J B Hanson a
PMCID: PMC366264  PMID: 16658329

Abstract

Determinations were made for corn (Zea mays L., WF9-Tms × M14) mitochondria of the stoichiometric relationship between K+ transport and bond energy produced in respiration (K+/∼ ratio). With inward pumping of potassium acetate activated by NADH oxidation, the initial rate of K+ transported into the sucrose inaccessible space varied between 0.58 and 0.97 K+/∼, assuming 2 high energy bond equivalents per NADH oxidized. Only small amounts of H+ were ejected. Valinomycin did not alter the ratio.

Efflux pumping of K+ in KCl media gave a K+/∼ of 0.92. Substituting sucrose for KCl caused a drop in respiration and H+ ejection, indicating a requirement for cation efflux pumping in acceptorless respiration. Valinomycin added to the KCl medium accelerated passive swelling but did not impair subsequent efflux pumping, although respiration was partially uncoupled.

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Selected References

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