Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1973 Feb;51(2):409–411. doi: 10.1104/pp.51.2.409

Possible Errors in Quantitative Determination of Phenylalanine Ammonia-Lyase Activity by Spectrophotometric Methods 1

A Erez a
PMCID: PMC366277  PMID: 16658342

Abstract

A possible error in spectrophotometric determination of cinnamate, the product of phenylalanine ammonia-lyase activity, using nonpurified protein extracts has been shown.

Under optimal conditions for phenylalanine ammonia-lyase activity, with borate buffer and in the presence of α-keto acids, phenylpyruvate is produced and its enol tautomer-borate complex formed, strongly absorbs at 290 nanometers.

Full text

PDF
409

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATFIELD G. N., MORRIS C. J. Analytical separations by highvoltage paper electrophoresis. Amino acids in protein hydrolysates. Biochem J. 1961 Dec;81:606–614. doi: 10.1042/bj0810606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bellini E., Hillman W. S. Red and far red effects on phenylalanine ammonia-lyase in raphanus and sinapis seedlings do not correlate with phytochrome spectrophotometry. Plant Physiol. 1971 May;47(5):668–671. doi: 10.1104/pp.47.5.668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Durst F., Mohr H. Phytochrome-mediated induction of enzyme synthesis in mustard seedlings(Sinapis alba L.). Naturwissenschaften. 1966 Oct;53(20):531–532. doi: 10.1007/BF00600655. [DOI] [PubMed] [Google Scholar]
  4. KNOX W. E., PITT B. M. Enzymic catalysis of the keto-enol tautomerization of phenylpyruvic acids. J Biol Chem. 1957 Apr;225(2):675–688. [PubMed] [Google Scholar]
  5. KOUKOL J., CONN E. E. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare. J Biol Chem. 1961 Oct;236:2692–2698. [PubMed] [Google Scholar]
  6. Scandurra R., Cannella C. A ssay of L-phenylalaine:2-oxoglutarate aminotransferase by determination of phenyl pyruvate. Anal Biochem. 1969 Feb;27(2):253–256. doi: 10.1016/0003-2697(69)90029-3. [DOI] [PubMed] [Google Scholar]
  7. Schopfer P., Mohr H. Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings: A Contribution to Eliminate Some Misconceptions. Plant Physiol. 1972 Jan;49(1):8–10. doi: 10.1104/pp.49.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Walton D. C., Sondheimer E. Effects of Abscisin II on Phenylalanine Ammonia-Lyase Activity in Excised Bean Axes. Plant Physiol. 1968 Mar;43(3):467–469. doi: 10.1104/pp.43.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Zucker M. Induction of Phenylalanine Deaminase by Light and its Relation to Chlorogenic Acid Synthesis in Potato Tuber Tissue. Plant Physiol. 1965 Sep;40(5):779–784. doi: 10.1104/pp.40.5.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Zucker M. Induction of phenylalanine ammonia-lyase in Xanthium leaf disks. Photosynthetic requirement and effect of daylength. Plant Physiol. 1969 Jun;44(6):912–922. doi: 10.1104/pp.44.6.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Zucker M. Sequential Induction of Phenylalanine Ammonia-lyase and a Lyase-inactivating System in Potato Tuber Disks. Plant Physiol. 1968 Mar;43(3):365–374. doi: 10.1104/pp.43.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES