Abstract
A cell-free, insoluble cell wall fraction is described which floats on the surface of suspension cultures of Glycine max L. Merrill var. Acme cells. Its accumulation is governed by both the shaking speed and the medium volume, a shaking speed of 110 to 120 revolutions per minute with a medium volume of about 100 to 120 milliliters in a 250-milliliter flask being optimal. Various factors which could control the accumulation of the complex were tested and are discussed, and scanning electron micrographs of the complex being released from the cell surface are presented.
The composition of the complex by weight is 46% galacturonic acid, 36% protein, 11% lignin (apparent), 4.4% arabinose, 2% ash, and 0.5% methyl ester. Evidence for an intimate relationship between the uronic acid and protein fractions is presented. The protein contains hydroxyproline, and the bulk of it is tightly bound to the complex, although a portion can be removed with high salt treatments.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aspinall G. O., Molloy J. A., Craig J. W. Extracellular polysaccharides from suspension-cultured sycamore cells. Can J Biochem. 1969 Nov;47(11):1063–1070. doi: 10.1139/o69-170. [DOI] [PubMed] [Google Scholar]
- Becker G. E., Hui P. A., Albersheim P. Synthesis of Extracellular Polysaccharide by Suspensions of Acer Pseudoplatanus Cells. Plant Physiol. 1964 Nov;39(6):913–920. doi: 10.1104/pp.39.6.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carceller M., Davey M. R., Fowler M. W., Street H. E. The influence of sucrose, 2,4-D, and kinetin on the growth, fine structure, and lignin content of cultured sycamore cells. Protoplasma. 1971;73(3):367–385. doi: 10.1007/BF01273940. [DOI] [PubMed] [Google Scholar]
- HOFSTEE B. H. Direct and continuous spectrophotometric assay of phosphomonoesterases. Arch Biochem Biophys. 1954 Jul;51(1):139–146. doi: 10.1016/0003-9861(54)90461-0. [DOI] [PubMed] [Google Scholar]
- Knutson C. A., Jeanes A. A new modification of the carbazole analysis: application to heteropolysaccharides. Anal Biochem. 1968 Sep;24(3):470–481. doi: 10.1016/0003-2697(68)90154-1. [DOI] [PubMed] [Google Scholar]
- LETHAM D. S. The separation of plant cells with ethylenediaminetetraacetic acid. Exp Cell Res. 1960 Nov;21:353–360. doi: 10.1016/0014-4827(60)90267-6. [DOI] [PubMed] [Google Scholar]
- Lamport D. T., Miller D. H. Hydroxyproline arabinosides in the plant kingdom. Plant Physiol. 1971 Oct;48(4):454–456. doi: 10.1104/pp.48.4.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NEUMAN R. E., LOGAN M. A. The determination of hydroxyproline. J Biol Chem. 1950 May;184(1):299–306. [PubMed] [Google Scholar]
- Olson A. C., Evans J. J., Frederick D. P., Jansen E. F. Plant suspension culture media macromolecules-pectic substances, protein, and peroxidase. Plant Physiol. 1969 Nov;44(11):1594–1600. doi: 10.1104/pp.44.11.1594. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts R. M. The metabolism of D-mannose- 14 C to polysaccharide in corn roots. Specific labeling of L-galactose, D-mannose, and L-fucose. Arch Biochem Biophys. 1971 Aug;145(2):685–692. doi: 10.1016/s0003-9861(71)80029-2. [DOI] [PubMed] [Google Scholar]
- Whitmore F. W. Lignin formation in wheat coleoptile cell walls: a possible limitation of cell growth. Plant Physiol. 1971 Nov;48(5):596–602. doi: 10.1104/pp.48.5.596. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wood P. J., Siddiqui I. R. Determination of methanol and its application to measurement of pectin ester content and pectin methyl esterase activity. Anal Biochem. 1971 Feb;39(2):418–428. doi: 10.1016/0003-2697(71)90432-5. [DOI] [PubMed] [Google Scholar]