Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1973 Apr;51(4):685–690. doi: 10.1104/pp.51.4.685

Further Studies on the Photosynthesis of Carrot Tissue Cultures 1

K-H Neumann a, A Raafat a,2
PMCID: PMC366329  PMID: 16658393

Abstract

The influence of kinetin and sucrose on the photosynthetic activity of carrot (Daucus carota) tissue cultures in relation to growth was investigated. The results showed that light contributes heavily to the growth of tissue cultures measured in terms of fresh and dry weight and cell division activity. In light, the fresh weight, dry weight, and number of cells per explant were about or more than doubled. This indicated that after the development of chloroplasts, carrot tissue cultures can grow autotrophically at least as far as energy and carbon are concerned. Kinetin was shown to have an important role in developing the photosynthetic apparatus and photosynthetic activity of tissue cultures as manifested by the increase of chlorophyll content (60%), Hill activity (about 3-fold), and 14C-fixation from NaH14CO3 (about 20%). On the other hand, the presence of sucrose in the medium reduced the chlorophyll content by about 30% and 14C-fixation from NaH14CO3 in the soluble fraction by about 60%. A possible correlation between the influence of kinetin on sugar uptake and the effect of kinetin on 14C-fixation from NaH14CO3 was discussed.

Full text

PDF
685

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVRON M., SHAVIT N. A SENSITIVE AND SIMPLE METHOD FOR DETERMINATION OF FERROCYANIDE. Anal Biochem. 1963 Dec;6:549–554. doi: 10.1016/0003-2697(63)90149-0. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gibbs M., Kandler O. ASYMMETRIC DISTRIBUTION OF C IN SUGARS FORMED DURING PHOTOSYNTHESIS. Proc Natl Acad Sci U S A. 1957 Jun 15;43(6):446–451. doi: 10.1073/pnas.43.6.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nobel P. S. A rapid technique for isolating chloroplasts with high rates of endogenous photophosphorylation. Plant Physiol. 1967 Oct;42(10):1389–1394. doi: 10.1104/pp.42.10.1389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Shugarman P. M., Appleman D. Chlorophyll Synthesis in Chlorella II. Effect of Glucose and Light Intensity on the Lag Phase. Plant Physiol. 1966 Dec;41(10):1701–1708. doi: 10.1104/pp.41.10.1701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stetler D. A., Laetsch W. M. Kinetin-induced chloroplast maturation in cultures of tobacco tissue. Science. 1965 Sep 17;149(3690):1387–1388. doi: 10.1126/science.149.3690.1387. [DOI] [PubMed] [Google Scholar]
  7. Treharne K. J., Stoddart J. L., Pughe J., Paranjothy K., Wareing P. F. Effects of gibberellin and cytokinins on the activity of photosynthetic enzymes and plastid ribosomal RNA synthesis in Phaseolus vulgaris L. Nature. 1970 Oct 10;228(5267):129–131. doi: 10.1038/228129a0. [DOI] [PubMed] [Google Scholar]
  8. Upper C. D., Helgeson J. P., Haberlach G. T. Limitation of tobacco callus tissue growth by carbohydrate availability. Plant Physiol. 1970 Jul;46(1):118–122. doi: 10.1104/pp.46.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES