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. 2012 Aug 31;3(9):1011–1025. doi: 10.18632/oncotarget.622

Figure 3. Effect of miR-31 overexpression on proliferation, migration, and invasion of melanoma cells.

Figure 3

A, qRT-PCR for miR-31 using total RNA from various melanoma cell lines after transient transfection of precursor miR-31 (pre-miR-31) or control miR. U6 snRNA serves as an endogenous control. Raw Ct values are plotted for both miR-31 and U6 snRNA. B, effect of miR-31 overexpression on proliferation in melanoma cell lines. Cells were quantitated by Coulter Counter at the indicated time points to monitor growth. C, effect of miR-31 overexpression on migration through Transwell and invasion through Matrigel in melanoma cell lines. Migrated and invaded cells were stained with crystal violet, solubilized and quantitated by measuring absorbance at 560 nm.* indicates p<0.05, Student's t-test.