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. 1973 May;51(5):863–867. doi: 10.1104/pp.51.5.863

Isocitrate Lyase in Green Leaves 1

H R Godavari a, S S Badour a, E R Waygood a
PMCID: PMC366364  PMID: 16658428

Abstract

Isocitrate lyase (EC 4.1.3.1) has been demonstrated in crude dialyzed extracts of healthy spinach (Spinacia oleracea) leaves from commercial sources and wheat (Triticum aestivum) and maize (Zea mays) leaves stored in darkness in the cold room for 1 week. The products of the reaction were identified as glyoxylate and succinate, the former by its phenylhydrazone, and the latter traced by isotopic labeling and cochromatography. Fresh spinach extracts contain a mixture of at least two endogenous inhibitors of isocitrate lyase activity and one of them is proteinaceous. The endogenous inhibitor(s) is thermostable and retains 50% of its inhibitory effect even after boiling for 10 minutes. Dark starvation of the leaves removes the inhibition, due possibly to autolysis of the inhibitor(s). The inhibitor(s) can also be removed by filtration through Sephadex gels. The crude extract from spinach shows double pH optima in phosphate buffer at pH 7.4 and pH 8.0. The apparent Km at pH 7.4 was 0.1 mm. Oxaloacetate, dl-malate, succinate, 3-phosphoglycerate, and glycolate at 10 mm concentration inhibited, but ribulose 1,5-diphosphate activated enzymic activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badour S. S., Waygood E. R. Glyoxylate carboxy-lyase activity in the unicellular green alga Gloeomonas sp. Biochim Biophys Acta. 1971 Aug 20;242(2):493–499. doi: 10.1016/0005-2744(71)90242-7. [DOI] [PubMed] [Google Scholar]
  2. Baechtel F. S., Hopkins H. A., Schmidt R. R. Continuous inducibility of isocitrate lyase during the cell cycle of the eucaryote Chlorella. Biochim Biophys Acta. 1970 Sep 17;217(1):216–219. doi: 10.1016/0005-2787(70)90144-9. [DOI] [PubMed] [Google Scholar]
  3. Beevers H. Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann N Y Acad Sci. 1969 Dec 19;168(2):313–324. doi: 10.1111/j.1749-6632.1969.tb43118.x. [DOI] [PubMed] [Google Scholar]
  4. Carpenter W. D., Beevers H. Distribution and Properties of Isocitritase in Plants. Plant Physiol. 1959 Jul;34(4):403–409. doi: 10.1104/pp.34.4.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerhardt B. P., Beevers H. Developmental studies on glyoxysomes in Ricinus endosperm. J Cell Biol. 1970 Jan;44(1):94–102. doi: 10.1083/jcb.44.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HAIGH W. G., BEEVERS H. THE OCCURENCE AND ASSAY OF ISOCITRATE LYASE IN ALGAE. Arch Biochem Biophys. 1964 Jul;107:147–151. doi: 10.1016/0003-9861(64)90282-6. [DOI] [PubMed] [Google Scholar]
  7. Harrop L. C., Kornberg H. L. The role of isocitrate lyase in the metabolism of algae. Proc R Soc Lond B Biol Sci. 1966 Nov 15;166(1002):11–29. doi: 10.1098/rspb.1966.0082. [DOI] [PubMed] [Google Scholar]
  8. KORNBERG H. L., BEEVERS H. A mechanism of conversion of fat to carbohydrate in castor beans. Nature. 1957 Jul 6;180(4575):35–36. doi: 10.1038/180035a0. [DOI] [PubMed] [Google Scholar]
  9. KORNBERG H. L., BEEVERS H. The glyoxylate cycle as a stage in the conversion of fat to carbohydrate in castor beans. Biochim Biophys Acta. 1957 Dec;26(3):531–537. doi: 10.1016/0006-3002(57)90101-4. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Longo G. P., Longo C. P. The development of glyoxysomes in maize scutellum: changes in morphology and enzyme compartmentation. Plant Physiol. 1970 Oct;46(4):599–604. doi: 10.1104/pp.46.4.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pearce J., Leach C. K., Carr N. G. The incomplete tricarboxylic acid cycle in the blue-green alga Anabaena variabilis. J Gen Microbiol. 1969 Mar;55(3):371–378. doi: 10.1099/00221287-55-3-371. [DOI] [PubMed] [Google Scholar]
  13. Rao G. R., McFadden B. A. Isocitrate lyase from Pseudomonas indigofera. IV. Specificity and inhibition. Arch Biochem Biophys. 1965 Nov;112(2):294–303. doi: 10.1016/0003-9861(65)90049-4. [DOI] [PubMed] [Google Scholar]
  14. Stoddart R. W., Northcote D. H. Metabolic relationships of the isolated fractions of the pectic substances of actively growing sycamore cells. Biochem J. 1967 Oct;105(1):45–59. doi: 10.1042/bj1050045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ZELITCH I. The role of glycolic acid oxidase in the respiration of leaves. J Biol Chem. 1958 Dec;233(6):1299–1303. [PubMed] [Google Scholar]

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