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. 1973 May;51(5):902–906. doi: 10.1104/pp.51.5.902

Characterization of Deoxyribonucleic Acid Species from Castor Bean Endosperm

Inability to Detect a Unique Deoxyribonucleic Acid Species Associated with Glyoxysomes 1

S A Douglass a, R S Criddle a, R W Breidenbach a
PMCID: PMC366373  PMID: 16658437

Abstract

Three DNA buoyant density species (nuclear, 1.692 g cm−3; mitochondria 1.705 g cm−3; and proplastid, 1.713 g cm−3) can be detected in extracts from castor bean endosperm. No other buoyant density species can be identified. DNA extracts from sucrose density gradient purified glyoxysomes exhibit varying amounts of each of the three identified DNAs but no other distinguishable DNA species. RNA synthesized in vitro by Escherichia coli RNA polymerase using purified castor bean nuclear DNA as a template, hybridizes equally well with its template and with the 1.692 g cm−3 species from glyoxysome fractions. These results are discussed in terms of their relevance to microbody biogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avers C. J., Federman M. The occurrence in yeast of cytoplasmic granules which resemble microbodies. J Cell Biol. 1968 May;37(2):555–559. doi: 10.1083/jcb.37.2.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernardi G., Faures M., Piperno G., Slonimski P. P. Mitochondrial DNA's from respiratory-sufficient and cytoplasmic respiratory-deficient mutant yeast. J Mol Biol. 1970 Feb 28;48(1):23–42. doi: 10.1016/0022-2836(70)90216-0. [DOI] [PubMed] [Google Scholar]
  3. Breidenbach R. W., Beevers H. Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm. Biochem Biophys Res Commun. 1967 May 25;27(4):462–469. doi: 10.1016/s0006-291x(67)80007-x. [DOI] [PubMed] [Google Scholar]
  4. Breidenbach R. W., Kahn A., Beevers H. Characterization of glyoxysomes from castor bean endosperm. Plant Physiol. 1968 May;43(5):705–713. doi: 10.1104/pp.43.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  6. Ching T. M. Glyoxysomes in megagamethophyte of germinating ponderosa pine seeds. Plant Physiol. 1970 Sep;46(3):475–482. doi: 10.1104/pp.46.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Church R. B., McCarthy B. J. Related base sequences in the DNA of simple and complex organisms. II. The interpretation of DNA-RNA hybridization studies with mammalian nucleic acids. Biochem Genet. 1968 Jun;2(1):55–73. doi: 10.1007/BF01458451. [DOI] [PubMed] [Google Scholar]
  8. Clark-Walker G. D. Isolation of circular DNA from a mitochondrial fraction from yeast. Proc Natl Acad Sci U S A. 1972 Feb;69(2):388–392. doi: 10.1073/pnas.69.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cohen S. N., Maitra U., Hurwitz J. Role of DNA in RNA synthesis. XI. Selective transcription of gamma DNA segments in vitro by RNA polymerase of Escherichia coli. J Mol Biol. 1967 May 28;26(1):19–38. doi: 10.1016/0022-2836(67)90258-6. [DOI] [PubMed] [Google Scholar]
  10. Gerhardt B. P., Beevers H. Occurrence of RNA in glyoxysomes from castor bean endosperm. Plant Physiol. 1969 Oct;44(10):1475–1477. doi: 10.1104/pp.44.10.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  12. Hoffmann H. P., Szabo A., Avers C. J. Cytochemical localization of catalase activity in yeast peroxisomes. J Bacteriol. 1970 Oct;104(1):581–584. doi: 10.1128/jb.104.1.581-584.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jagendorf A. T., Wildman S. G. The Proteins of Green Leaves. VI. Centrifugal Fractionation of Tobacco Leaf Homogenates and Some Properties of Isolated Chloroplasts. Plant Physiol. 1954 May;29(3):270–279. doi: 10.1104/pp.29.3.270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Michaelis G., Douglass S., Tsai M. J., Burchiel K., Criddle R. S. In vitro transcription of mitochondrial deoxyribonucleic acid from yeast. Biochemistry. 1972 May 23;11(11):2026–2036. doi: 10.1021/bi00761a006. [DOI] [PubMed] [Google Scholar]
  16. Raison J. K., Lyons J. M., Thomson W. W. The influence of membranes on the temperature-induced changes in the kinetics of some respiratory enzymes of mitochondria. Arch Biochem Biophys. 1971 Jan;142(1):83–90. doi: 10.1016/0003-9861(71)90261-x. [DOI] [PubMed] [Google Scholar]
  17. Suyama Y., Bonner W. D. DNA from plant mitochondria. Plant Physiol. 1966 Mar;41(3):383–388. doi: 10.1104/pp.41.3.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Szabo A. S., Avers C. J. Some aspects of regulation of peroxisomes and mitochondria in yeast. Ann N Y Acad Sci. 1969 Dec 19;168(2):302–312. doi: 10.1111/j.1749-6632.1969.tb43117.x. [DOI] [PubMed] [Google Scholar]
  19. Tsai M. J., Michaelis G., Criddle R. S. DNA-dependent RNA polymerase from yeast mitochondria. Proc Natl Acad Sci U S A. 1971 Feb;68(2):473–477. doi: 10.1073/pnas.68.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vigil E. L. Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J Cell Biol. 1970 Sep;46(3):435–454. doi: 10.1083/jcb.46.3.435. [DOI] [PMC free article] [PubMed] [Google Scholar]

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