Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1973 May;51(5):907–913. doi: 10.1104/pp.51.5.907

Mechanism of Naphthaleneacetic Acid Conjugation

No Effect of Ethylene 1

R Goren a,2, Martin J Bukovac a
PMCID: PMC366374  PMID: 16658438

Abstract

Formation of naphthaleneacetic acid-glucose (NAGLu) in detached leaves, floating on α-naphthaleneacetic acid-1-14C (NAA, 0.05 microcurie per milliliter, 3.1 μm)-buffer solution (phosphate-citrate, pH 4.2) began immediately while there was a 2- to 4-hour lag before NAA-asparatate (NAAsp) could be detected. Subsequent increase in the NAAsp conjugate reflected a decrease in free NAA to 1 to 2% of the total radioactivity taken up. Pretreatment with 31 μm12C-NAA for 18 hours doubled NAAsp formation after transfer for 4 hours to 14C-NAA. Pretreatment with ethylene, as ethephon (up to 400 milligrams per liter) or ethylene gas (10 microliters per liter), did not induce NAAsp formation. In the presence of NAA, ethylene had no effect on NAA conjugation. Similarly, CO2 (5%) did not modify the formation of the conjugates. Rhizobitoxine (1.87 μm) inhibited NAA-induced ethylene production but did not prevent NAA-induced formation of NAAsp. We concluded that the conjugation of NAA with aspartic acid is not mediated by ethylene.

Full text

PDF
907

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andreae W. A., Robinson J. R., Van Ysselstein M. W. Studies on 3-indoleacetic acid metabolism. VII. Metabolism of radioactive 3-indoleacetic acid by pea roots. Plant Physiol. 1961 Nov;36(6):783–787. doi: 10.1104/pp.36.6.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andreae W. A., Van Ysselstein M. W. Studies on 3-Indoleacetic Acid Metabolism. VI. 3-Indoleacetic Acid Uptake and Metabolism by Pea Roots and Epicotyls. Plant Physiol. 1960 Mar;35(2):225–232. doi: 10.1104/pp.35.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Andreae W. A., Ysselstein M. W. Studies on 3-Indoleacetic Acid Metabolism. III. The Uptake of 3-Indoleacetic Acid by Pea Epicotyls and Its Conversion to 3-Indoleacetylaspartic Acid. Plant Physiol. 1956 May;31(3):235–240. doi: 10.1104/pp.31.3.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beyer E. M., Morgan P. W. Effect of ethylene on the uptake, distribution, and metabolism of indoleacetic Acid-1-C and -2-C and naphthaleneacetic Acid-1-C. Plant Physiol. 1970 Jul;46(1):157–162. doi: 10.1104/pp.46.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burg S. P., Burg E. A. Ethylene formation in pea seedlings; its relation to the inhibition of bud growth caused by indole-3-acetic Acid. Plant Physiol. 1968 Jul;43(7):1069–1074. doi: 10.1104/pp.43.7.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burg S. P., Burg E. A. Molecular requirements for the biological activity of ethylene. Plant Physiol. 1967 Jan;42(1):144–152. doi: 10.1104/pp.42.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chadwick A. V., Burg S. P. Regulation of root growth by auxin-ethylene interaction. Plant Physiol. 1970 Feb;45(2):192–200. doi: 10.1104/pp.45.2.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Craker L. E., Chadwick A. V., Leather G. R. Abscission: movement and conjugation of auxin. Plant Physiol. 1970 Dec;46(6):790–793. doi: 10.1104/pp.46.6.790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ernest L. C., Valdovinos J. G. Regulation of Auxin Levels in Coleus blumei by Ethylene. Plant Physiol. 1971 Oct;48(4):402–406. doi: 10.1104/pp.48.4.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Good N. E., Andreae W. A., Ysselstein M. W. Studies on 3-Indoleacetic Acid Metabolism. II. Some Products of the Metabolism of Exogenous Indoleacetic Acid in Plant Tissues. Plant Physiol. 1956 May;31(3):231–235. doi: 10.1104/pp.31.3.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kang B. G., Newcomb W., Burg S. P. Mechanism of Auxin-induced Ethylene Production. Plant Physiol. 1971 Apr;47(4):504–509. doi: 10.1104/pp.47.4.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morgan P. W., Gausman H. W. Effects of ethylene on auxin transport. Plant Physiol. 1966 Jan;41(1):45–52. doi: 10.1104/pp.41.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Olney H. O. Growth substances from Veratrum tenuipetalum. Plant Physiol. 1968 Mar;43(3):293–302. doi: 10.1104/pp.43.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Riov J., Monselise S. P., Kahan R. S. Ethylene-controlled Induction of Phenylalanine Ammonia-lyase in Citrus Fruit Peel. Plant Physiol. 1969 May;44(5):631–635. doi: 10.1104/pp.44.5.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Venis M. A. Auxin-induced Conjugation Systems in Peas. Plant Physiol. 1972 Jan;49(1):24–27. doi: 10.1104/pp.49.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Warner H. L., Leopold A. C. Ethylene evolution from 2-chloroethylphosphonic Acid. Plant Physiol. 1969 Jan;44(1):156–158. doi: 10.1104/pp.44.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ZENK M. H. I-(Indole-3-acetyl)-beta-D-glucose, a new compound in the metabolism of indole-3-acetic acid in plants. Nature. 1961 Jul 29;191:493–494. doi: 10.1038/191493a0. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES