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. 1973 May;51(5):939–945. doi: 10.1104/pp.51.5.939

Immunochemistry of Phytochrome 1

Harbert V Rice a,2, Winslow R Briggs a
PMCID: PMC366378  PMID: 16658442

Abstract

Rabbit antibody was elicited against purified oat phytochrome polypeptides. Immunodiffusion and immunoelectrophoresis indicated the antibody elicited was predominantly a single precipitin system. No antigenic difference was detected between red-absorbing phytochrome and far red-absorbing phytochrome. Crude preparations of rye and corn phytochrome showed a line of identity when cross-reacted with oat polypeptide phytochrome; pea phytochrome showed a line of partial identity. Precipitin reactions with purified rye phytochrome analyzed with sucrose density gradient centrifugation and immunodiffusion confirmed that the same class of determinants was available to the antibody when the protein was known to be in a state which had not undergone extensive proteolytic attack.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson G. R., Jenner E. L., Mumford F. E. Temperature and pH studies on phytochrome in vitro. Biochemistry. 1969 Mar;8(3):1182–1187. doi: 10.1021/bi00831a052. [DOI] [PubMed] [Google Scholar]
  2. BROWN R. K., TACEY B. C., ANFINSEN C. B., Jr The reaction of porcine and ovine ribonucleases with antibody to bovine ribonuclease. Biochim Biophys Acta. 1960 Apr 22;39:528–530. doi: 10.1016/0006-3002(60)90207-9. [DOI] [PubMed] [Google Scholar]
  3. BUTLER W. L., SIEGELMAN H. W., MILLER C. O. DENATURATION OF PHYTOCHROME. Biochemistry. 1964 Jun;3:851–857. doi: 10.1021/bi00894a022. [DOI] [PubMed] [Google Scholar]
  4. Berns D. S. Immunochemistry of biliproteins. Plant Physiol. 1967 Nov;42(11):1569–1586. doi: 10.1104/pp.42.11.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Briggs W. R., Zollinger W. D., Platz B. B. Some Properties of Phytochrome Isolated From Dark-grown Oat Seedlings (Avena sativa L.). Plant Physiol. 1968 Aug;43(8):1239–1243. doi: 10.1104/pp.43.8.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Butler W. L., Lane H. C. Dark Transformations of Phytochrome in vivo. II. Plant Physiol. 1965 Jan;40(1):13–17. doi: 10.1104/pp.40.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Butler W. L., Norris K. H., Siegelman H. W., Hendricks S. B. DETECTION, ASSAY, AND PRELIMINARY PURIFICATION OF THE PIGMENT CONTROLLING PHOTORESPONSIVE DEVELOPMENT OF PLANTS. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1703–1708. doi: 10.1073/pnas.45.12.1703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Correll D. L., Steers E., Jr, Towe K. M., Shropshire W., Jr Phytochrome in etiolated annual rye. IV. Physical and chemical characterization of phytochrome. Biochim Biophys Acta. 1968 Sep 10;168(1):46–57. doi: 10.1016/0005-2795(68)90232-8. [DOI] [PubMed] [Google Scholar]
  9. Furuya M., Hillman W. S. Rapid Destruction of the P(FS) Form of Phytochrome by a Substance in Extracts of Pisum Tissue. Plant Physiol. 1966 Sep;41(7):1242–1244. doi: 10.1104/pp.41.7.1242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gardner G., Pike C. S., Rice H. V., Briggs W. R. "Disaggregation" of phytochrome in vitro-a consequence of proteolysis. Plant Physiol. 1971 Dec;48(6):686–693. doi: 10.1104/pp.48.6.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hopkins D. W., Butler W. L. Immunochemical and spectroscopic evidence for protein conformational changes in phytochrome transformations. Plant Physiol. 1970 May;45(5):567–570. doi: 10.1104/pp.45.5.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  13. Mumford F. E. Studies on the phytochrome dark reaction in vitro. Biochemistry. 1966 Feb;5(2):522–524. doi: 10.1021/bi00866a018. [DOI] [PubMed] [Google Scholar]
  14. PORTER R. R. The isolation and properties of a fragment of bovine-serum albumin which retains the ability to combine with rabbit antiserum. Biochem J. 1957 Aug;66(4):677–686. doi: 10.1042/bj0660677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pike C. S., Briggs W. R. The dark reactions of rye phytochrome in vivo and in vitro. Plant Physiol. 1972 Apr;49(4):514–520. doi: 10.1104/pp.49.4.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pringle J. R. The molecular weight of the undegraded polypeptide chain of yeast hexokinase. Biochem Biophys Res Commun. 1970 Apr 8;39(1):46–52. doi: 10.1016/0006-291x(70)90755-2. [DOI] [PubMed] [Google Scholar]
  17. Purves W. K., Briggs W. R. Kinetically distinguishable populations of phytochrome. Plant Physiol. 1968 Aug;43(8):1259–1263. doi: 10.1104/pp.43.8.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rice H. V., Briggs W. R., Jackson-White C. J. Purification of oat and rye phytochrome. Plant Physiol. 1973 May;51(5):917–926. doi: 10.1104/pp.51.5.917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rice H. V., Briggs W. R. Partial characterization of oat and rye phytochrome. Plant Physiol. 1973 May;51(5):927–938. doi: 10.1104/pp.51.5.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Roux S. J., Hillman W. S. The effect of glutaraldehyde and two monoaldehydes on phytochrome. Arch Biochem Biophys. 1969 May;131(2):423–429. doi: 10.1016/0003-9861(69)90414-7. [DOI] [PubMed] [Google Scholar]
  21. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  22. Taylor A. O. In vitro phytochrome dark reversion process. Plant Physiol. 1968 May;43(5):767–774. doi: 10.1104/pp.43.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

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