Abstract
Linear increments in ferulic acid concentration produce logarithmic increases in the ferulic acid-induced lag periods prior to the peroxidase-catalyzed oxidation of indole-3-acetic acid in a system containing 2,4-dichlorophenol and MnCl2 in acetate buffer at pH 5.6. Maintaining the ratio of indole-3-acetic acid to ferulic acid constant at 100 while linearly raising the ferulic acid concentration results in linear increases in the lag period. Both indole-3-acetic acid and ferulic acid are substrates of horseradish peroxidase in the presence of H2O2, and indole-3-acetic acid competitively inhibits the oxidation of ferulic acid. A model for the enzymatic oxidation of indole-3-acetic acid catalyzed by peroxidase is proposed.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ENGELSMA G. DESTRUCTION OF HYDROXYCINNAMIC ACIDS COUPLED TO OXIDATION OF INDOLYL-3-ACETIC ACID BY PEROXIDASE AS A POSSIBLE MECHANISM FOR ADAPTIVE CHANGES OF INDOLYL-3-ACETIC ACID OXIDASE ACTIVITY. Nature. 1964 Apr 4;202:88–89. doi: 10.1038/202088a0. [DOI] [PubMed] [Google Scholar]
- Fox L. R., Purves W. K., Nakada H. I. The role of horseradish peroxidase in indole-3-acetic acid oxidation. Biochemistry. 1965 Dec;4(12):2754–2763. doi: 10.1021/bi00888a028. [DOI] [PubMed] [Google Scholar]
- Gelinas D., Postlethwait S. N. IAA Oxidase Inhibitors from Normal and Mutant Maize Plants. Plant Physiol. 1969 Nov;44(11):1553–1559. doi: 10.1104/pp.44.11.1553. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gordon S. A., Weber R. P. COLORIMETRIC ESTIMATION OF INDOLEACETIC ACID. Plant Physiol. 1951 Jan;26(1):192–195. doi: 10.1104/pp.26.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HINMAN R. L., LANG J. PEROXIDASE-CATALYZED OXIDATION OF INDOLE-3-ACETIC ACID. Biochemistry. 1965 Jan;4:144–158. doi: 10.1021/bi00877a023. [DOI] [PubMed] [Google Scholar]
- MUDD J. B., BURRIS R. H. Inhibition of peroxidase-catalyzed oxidations. J Biol Chem. 1959 Dec;234:3281–3285. [PubMed] [Google Scholar]
- Meudt W. J., Gaines T. P. Studies on the oxidation of indole-3-acetic Acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic Acid oxidation products. Plant Physiol. 1967 Oct;42(10):1395–1399. doi: 10.1104/pp.42.10.1395. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller R. W., Parups E. V. The effect of 2,2-diphenyl-1-picrylhydrazyl and p-cresol on the oxidative degradation of indole-3-acetate. Arch Biochem Biophys. 1971 Mar;143(1):276–285. doi: 10.1016/0003-9861(71)90210-4. [DOI] [PubMed] [Google Scholar]
- RAY P. M. Destruction of indoleacetic acid. IV. Kinetics of enzymic oxidation. Arch Biochem Biophys. 1962 Feb;96:199–209. doi: 10.1016/0003-9861(62)90399-5. [DOI] [PubMed] [Google Scholar]
- RAY P. M. The destruction of indoleacetic acid. II. Spectrophotometric study of the enzymatic reaction. Arch Biochem Biophys. 1956 Sep;64(1):193–216. doi: 10.1016/0003-9861(56)90254-5. [DOI] [PubMed] [Google Scholar]
- Ricard J., Nari J. The formation and reactivity of peroxidase compound 3. Biochim Biophys Acta. 1967 Mar 15;132(2):321–329. doi: 10.1016/0005-2744(67)90151-9. [DOI] [PubMed] [Google Scholar]
- Sequeira L., Mineo L. Partial purification and kinetics of indoleacetic Acid oxidase from tobacco roots. Plant Physiol. 1966 Sep;41(7):1200–1208. doi: 10.1104/pp.41.7.1200. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sirois J. C., Miller R. W. The Mechanism of the Scopoletin-induced Inhibition of the Peroxidase Catalyzed Degradation of Indole-3-acetate. Plant Physiol. 1972 Jun;49(6):1012–1018. doi: 10.1104/pp.49.6.1012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stonier T., Singer R. W., Yang H. M. Studies on Auxin Protectors: IX. Inactivation of Certain Protectors by Polyphenol Oxidase. Plant Physiol. 1970 Sep;46(3):454–457. doi: 10.1104/pp.46.3.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stonier T., Yoneda Y., Rodriguez-Tormes F. Studies on Auxin Protectors. V. On the Mechanism of IAA Protection by Protector-I of the Japanese Morning Glory. Plant Physiol. 1968 Jul;43(7):1141–1145. doi: 10.1104/pp.43.7.1141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yokota K., Yamazaki I. Reaction of peroxidase with reduced nicotinamide-adenine dinucleotide and reduced nicotinamide-adenine dinucleotide phosphate. Biochim Biophys Acta. 1965 Aug 24;105(2):301–312. doi: 10.1016/s0926-6593(65)80154-0. [DOI] [PubMed] [Google Scholar]