Abstract
Soluble proteins from excised Phaseolus vulgaris axes incubated for 1 hour in 3H or 14C- amino acid mixtures at different times during the period leading up to initiation of cell elongation were compared by acrylamide gel electrophoresis. Differences in electrophoretic patterns were found when proteins from axes incubated during the 1st hour of imbibition were compared with proteins from axes incubated during the hour when cell elongation was initiated. These differences greatly diminished by the 2nd hour of imbibition which suggests that they were due primarily to incomplete axis imbibition. A 5-hour actinomycin D treatment which reduced amino acid incorporation by 40% in the 5th hour had no apparent effect on the electrophoretic pattern during that hour.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chen D., Sarid S., Katchalski E. Studies on the nature of messenger RNA in germinating wheat embryos. Proc Natl Acad Sci U S A. 1968 Jul;60(3):902–909. doi: 10.1073/pnas.60.3.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Marcus A. Seed germination and the capacity for protein synthesis. Symp Soc Exp Biol. 1969;23:143–160. [PubMed] [Google Scholar]
- Walton D. C. Germination of phaseolus vulgaris I. Resumption of axis growth. Plant Physiol. 1966 Feb;41(2):298–302. doi: 10.1104/pp.41.2.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waters L. C., Dure L. S., 3rd Ribonucleic acid synthesis in germinating cotton seeds. J Mol Biol. 1966 Aug;19(1):1–27. doi: 10.1016/s0022-2836(66)80046-3. [DOI] [PubMed] [Google Scholar]
- Weeks D. P., Marcus A. Preformed messenger of quiescent wheat embryos. Biochim Biophys Acta. 1971 Apr 8;232(4):671–684. doi: 10.1016/0005-2787(71)90759-3. [DOI] [PubMed] [Google Scholar]