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. 1973 Aug;52(2):156–161. doi: 10.1104/pp.52.2.156

Fat Metabolism in Higher Plants

LVII. A Comparison of Fatty Acid-Synthesizing Enzymes in Chloroplasts Isolated from Mature and Immature Leaves of Spinach 1

C Gamini Kannangara a, Bruce S Jacobson a, P K Stumpf a
PMCID: PMC366459  PMID: 16658518

Abstract

Chloroplasts isolated from immature leaves of spinach (Spinacia oleracea) differ in enzyme levels from those isolated from mature leaves. On a chlorophyll basis, immature chloroplast preparations had 5- to 6-fold higher capacity to synthesize fatty acids from 2-14C-acetate compared to plastids isolated from mature leaves. This difference was correlated with higher activities for the enzymes, acetyl coenzyme A synthetase, malonyl coenzyme A synthetase, acetyl coenzyme A carboxylase, and oleyl coenzyme A transferase in plastid pressates obtained from immature leaves. Disrupted chloroplast preparations from both mature and immature leaves retained the ability to incorporate 2-14C-acetate into fatty acids in a pattern similar to that by isolated chloroplasts. 2-14C-Acetate, 2-14C-acetyl coenzyme A, 2-14C-malonate, and 1,3-14C malonyl coenzyme A were readily incorporated into a number of fatty acids. Moreover, the synthesis of oleate by chloroplast pressates from these substrates was strongly inhibited by KCN, flavin adenine mononucleotides and dinucleotides, and anaerobic conditions, while linolenic acid synthesis was unaffected by these compounds.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. BRUINSMA J. A comment on the spectrophotometric determination of chlorophyll. Biochim Biophys Acta. 1961 Sep 30;52:576–578. doi: 10.1016/0006-3002(61)90418-8. [DOI] [PubMed] [Google Scholar]
  3. Brooks J. L., Stumpf P. K. Fat metabolism in higher plants. XXXIX. Properties of a soluble fatty acid synthesizing system from lettuce chloroplasts. Arch Biochem Biophys. 1966 Sep 26;116(1):108–116. doi: 10.1016/0003-9861(66)90019-1. [DOI] [PubMed] [Google Scholar]
  4. GOLDMAN P., ALBERTS A. W., VAGELOS P. R. The condensation reaction of fatty acid biosynthesis. II. Requirement of the enzymes of the condensation reaction for fatty acid synthesis. J Biol Chem. 1963 Apr;238:1255–1261. [PubMed] [Google Scholar]
  5. Givan C. V., Stumpf P. K. Fat Metabolism in Higher Plants: XLV. Some Factors Regulating Fatty Acid Synthesis by Isolated Spinach Chloroplasts. Plant Physiol. 1971 Apr;47(4):510–515. doi: 10.1104/pp.47.4.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harwood J. L., Stumpf P. K. Fat metabolism in higher plants. 43. Control of fatty acid synthesis in germinating seeds. Arch Biochem Biophys. 1971 Jan;142(1):281–291. doi: 10.1016/0003-9861(71)90285-2. [DOI] [PubMed] [Google Scholar]
  7. Hitchcock C., James A. T. The mechanism of alpha-oxidation in leaves. Biochim Biophys Acta. 1966 Jun 1;116(3):413–424. doi: 10.1016/0005-2760(66)90112-3. [DOI] [PubMed] [Google Scholar]
  8. Huang K. P. A sensitive assay method of acetyl CoA synthetase. Anal Biochem. 1970 Sep;37(1):98–104. doi: 10.1016/0003-2697(70)90263-0. [DOI] [PubMed] [Google Scholar]
  9. Jacobson B. S., Kannangara C. G., Stumpf P. K. Biosynthesis of -linolenic acid by disrupted spinach chloroplasts. Biochem Biophys Res Commun. 1973 Mar 17;51(2):487–493. doi: 10.1016/0006-291x(73)91283-7. [DOI] [PubMed] [Google Scholar]
  10. Jacobson B. S., Stumpf P. K. Fat metabolism in higher plants. LV. Acetate uptake and accumulation by class I and II chloroplasts from Spinacia oleracea. Arch Biochem Biophys. 1972 Dec;153(2):656–663. doi: 10.1016/0003-9861(72)90384-0. [DOI] [PubMed] [Google Scholar]
  11. Kannangara C. G., Jacobson B. S., Stumpf P. K. In vivo biosynthesis of -linolenic acid in plants. Biochem Biophys Res Commun. 1973 May 15;52(2):648–655. doi: 10.1016/0006-291x(73)90762-6. [DOI] [PubMed] [Google Scholar]
  12. Kannangara C. G., Stumpf P. K. Fat Metabolism in Higher Plants: XLVII. The Effect of Nitrite and Other Anions on the Formation of Unsaturated Fatty Acids by Isolated Chloroplasts. Plant Physiol. 1972 Apr;49(4):497–501. doi: 10.1104/pp.49.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kannangara C. G., Stumpf P. K. Fat metabolism in higher plants. I. The biosynthesis of polyunsaturated fatty acids by isolated spinach chloroplasts. Arch Biochem Biophys. 1972 Feb;148(2):414–424. doi: 10.1016/0003-9861(72)90159-2. [DOI] [PubMed] [Google Scholar]
  14. Kannangara C. G., Stumpf P. K. Fat metabolism in higher plants. LIV. A procaryotic type acetyl CoA carboxylase in spinach chloroplasts. Arch Biochem Biophys. 1972 Sep;152(1):83–91. doi: 10.1016/0003-9861(72)90196-8. [DOI] [PubMed] [Google Scholar]
  15. Kannangara C. G., Stumpf P. K. Fat metabolism in higher plants. LVI. Distribution and nature of biotin in chloroplasts of different plant species. Arch Biochem Biophys. 1973 Apr;155(2):391–399. doi: 10.1016/0003-9861(73)90128-8. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
  18. MUDD J. B., MCMANUS T. T. RELATIONSHIP OF THE SYNTHESES OF LIPID AND WATER SOLUBLE ACIDS BY CHLOROPLAST PREPARATIONS. Plant Physiol. 1965 Mar;40:340–344. doi: 10.1104/pp.40.2.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MUDD J. B., McMANUS T. T. Metabolism of acetate by cellfree preparations from spinach leaves. J Biol Chem. 1962 Jul;237:2057–2063. [PubMed] [Google Scholar]
  20. Mudd J. B., McManus T. T. Participation of Sulfhydryl Groups in Fatty Acid Synthesis by Chloroplast Preparations. Plant Physiol. 1964 Jan;39(1):115–119. doi: 10.1104/pp.39.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. STUMPF P. K., JAMES A. T. The biosynthesis of long-chain fatty acids by lettuce chloroplast preparations. Biochim Biophys Acta. 1963 Feb 19;70:20–32. doi: 10.1016/0006-3002(63)90715-7. [DOI] [PubMed] [Google Scholar]
  22. Stumpf P. K., Boardman N. K. Fat metabolism in higher plants. XXXIX. Effect of adenosine triphosphate and triton X-100 on lipid synthesis by isolated spinach chloroplasts. J Biol Chem. 1970 May 25;245(10):2579–2587. [PubMed] [Google Scholar]

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