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. 1973 Sep;52(3):208–214. doi: 10.1104/pp.52.3.208

Hormonal Control of Orthophosphate Incorporation into Phospholipids of Barley Aleurone Layers 1

D E Koehler a,2, J E Varner a
PMCID: PMC366471  PMID: 16658533

Abstract

Gibberellic acid added to isolated barley aleurone layers enhances orthophosphate incorporation into chloroform-methanol-soluble compounds. The effect is measurable at 4 to 6 hours after the addition of gibberellic acid and reaches a maximum after 8 to 12 hours. The increase in the rate of orthophosphate incorporation is 3- to 5-fold over the rate in control layers incubated without gibberellic acid.

The gibberellic acid enhancement of the rate of phospholipid labeling is inhibited within 1 to 2 hours by cycloheximide, 6-methylpurine, and abscisic acid.

The increase in labeling of phospholipids occurs throughout the subcellular fractions rather than being restricted to a specific fraction or organelle. The increase in radioactivity in phospholipids as shown by thin layer chromatography is due to a proportional increase in all phospholipids.

The enhancement by gibberellic acid of the rate of phospholipid labeling may be required for the subsequent production of gibberellic acid-induced hydrolases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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