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. 1973 Sep;52(3):208–214. doi: 10.1104/pp.52.3.208

Hormonal Control of Orthophosphate Incorporation into Phospholipids of Barley Aleurone Layers 1

D E Koehler a,2, J E Varner a
PMCID: PMC366471  PMID: 16658533

Abstract

Gibberellic acid added to isolated barley aleurone layers enhances orthophosphate incorporation into chloroform-methanol-soluble compounds. The effect is measurable at 4 to 6 hours after the addition of gibberellic acid and reaches a maximum after 8 to 12 hours. The increase in the rate of orthophosphate incorporation is 3- to 5-fold over the rate in control layers incubated without gibberellic acid.

The gibberellic acid enhancement of the rate of phospholipid labeling is inhibited within 1 to 2 hours by cycloheximide, 6-methylpurine, and abscisic acid.

The increase in labeling of phospholipids occurs throughout the subcellular fractions rather than being restricted to a specific fraction or organelle. The increase in radioactivity in phospholipids as shown by thin layer chromatography is due to a proportional increase in all phospholipids.

The enhancement by gibberellic acid of the rate of phospholipid labeling may be required for the subsequent production of gibberellic acid-induced hydrolases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chrispeels M. J., Varner J. E. Hormonal control of enzyme synthesis: on the mode of action of gibberellic Acid and abscisin in aleurone layers of barley. Plant Physiol. 1967 Jul;42(7):1008–1016. doi: 10.1104/pp.42.7.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins G. G., Jenner C. F., Paleg L. G. The metabolism of soluble nucleotides in wheat aleurone layers treated with gibberellic Acid. Plant Physiol. 1972 Mar;49(3):404–410. doi: 10.1104/pp.49.3.404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evins W. H., Varner J. E. Hormone-controlled synthesis of endoplasmic reticulum in barley aleurone cells. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1631–1633. doi: 10.1073/pnas.68.7.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson K. D., Kende H. Hormonal Control of Lecithin Synthesis in Barley Aleurone Cells: Regulation of the CDP-Choline Pathway by Gibberellin. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2674–2677. doi: 10.1073/pnas.68.11.2674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones R. L. Inhibition of Gibberellic Acid-induced alpha-Amylase Formation by Polyethylene Glycol and Mannitol. Plant Physiol. 1969 Jan;44(1):101–104. doi: 10.1104/pp.44.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
  9. Paleg L. G. Physiological Effects of Gibberellic Acid: I. On Carbohydrate Metabolism and Amylase Activity of Barley Endosperm. Plant Physiol. 1960 May;35(3):293–299. doi: 10.1104/pp.35.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saha S., Good N. E. Products of the photophosphorylation reaction. J Biol Chem. 1970 Oct 10;245(19):5017–5021. [PubMed] [Google Scholar]

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