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. 1973 Sep;52(3):233–235. doi: 10.1104/pp.52.3.233

Cholinesterases from Plant Tissues

II. Inhibition of Bean Cholinesterase by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride (AMO-1618) 1

J Riov a,2, M J Jaffe a
PMCID: PMC366475  PMID: 16658537

Abstract

2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl chloride (AMO-1618) inhibits the activity of a cholinesterase isolated from mung bean (Phaseolus aureus) roots at concentrations comparable to those which retard growth and inhibit development of secondary roots of bean seedlings. Fifty per cent inhibition of the cholinesterase activity occurred at 0.21 mm. Inhibition of the cholinesterase is non-competitive and irreversible. The growth retardant seems to be a specific cholinesterase inhibitor since it has no effect on non-choline esterases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davies J. H., Campbell W. R., Kearns C. W. Inhibition of fly head acetylcholinesterase by bis-[(m-hydroxyphenyl)-trimethylammonium iodide] esters of polymethylenedicarbamic acids. Biochem J. 1970 Apr;117(2):221–230. doi: 10.1042/bj1170221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ELLMAN G. L., COURTNEY K. D., ANDRES V., Jr, FEATHER-STONE R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 Jul;7:88–95. doi: 10.1016/0006-2952(61)90145-9. [DOI] [PubMed] [Google Scholar]
  3. Greenspan C. M., Wilson I. B. The effect of fluoride on the reaction of acetylcholinesterase with carbamates. Mol Pharmacol. 1970 May;6(3):266–272. [PubMed] [Google Scholar]
  4. Jaffe M. J. Evidence for the regulation of phytochrome-mediated processes in bean roots by the neurohumor, acetylcholine. Plant Physiol. 1970 Dec;46(6):768–777. doi: 10.1104/pp.46.6.768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ooms A. J., Breebaart-Hansen J. C., Ceulen B. I. The reaction of organophosphorus compounds with hydrolytic enzymes. II. The inhibition of citrus acetylesterase. Biochem Pharmacol. 1966 Jan;15(1):17–30. doi: 10.1016/0006-2952(66)90106-7. [DOI] [PubMed] [Google Scholar]
  6. Riov J., Jaffe M. J. Cholinesterases from plant tissues: I. Purification and characterization of a cholinesterase from mung bean roots. Plant Physiol. 1973 Mar;51(3):520–528. doi: 10.1104/pp.51.3.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. WILSON I. B., HARRISON M. A., GINSBURG S. Carbamyl derivatives of acetylcholinesterase. J Biol Chem. 1961 May;236:1498–1500. [PubMed] [Google Scholar]
  8. WILSON I. B., HATCH M. A., GINSBURG S. Carbamylation of acetvlcholinesterase. J Biol Chem. 1960 Aug;235:2312–2315. [PubMed] [Google Scholar]

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