Abstract
Two polygalacturonases (PG I and PG II) have been separated from extracts of ripe peaches (Prunus persica) by chromatography on Sephadex G-100. PG I hydrolyzes polygalacturonic acid from the nonreducing ends of the molecules, releasing galacturonic acid as the product. It functions optimally at pH 5.5, requires Ca2+ for activity, and hydrolyzes low molecular weight substrates most rapidly. In contrast, PG II cleaves the molecular chain of the substrate randomly with a pH optimum at about 4. This enzyme is most reactive with substrates of intermediate molecular weight. It catalyzes the release of water-soluble, but 70% ethanol-insoluble, pectin from washed peach cell walls.
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Selected References
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- ASH A. S., REYNOLDS T. M. Watersoluble constituents of fruit. I. Some observations on the occurrence of free galacturonic acid in fruit. Aust J Biol Sci. 1954 Nov;7(4):435–443. doi: 10.1071/bi9540435. [DOI] [PubMed] [Google Scholar]
- English P. D., Maglothin A., Keegstra K., Albersheim P. A Cell Wall-degrading Endopolygalacturonase Secreted by Colletotrichum lindemuthianum. Plant Physiol. 1972 Mar;49(3):293–298. doi: 10.1104/pp.49.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karr A. L., Albersheim P. Polysaccharide-degrading Enzymes are Unable to Attack Plant Cell Walls without Prior Action by a "Wall-modifying Enzyme". Plant Physiol. 1970 Jul;46(1):69–80. doi: 10.1104/pp.46.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCREADY R. M., SEEGMILLER C. G. Action of pectic enzymes on oligogalacturonic acids and some of their derivatives. Arch Biochem Biophys. 1954 Jun;50(2):440–450. doi: 10.1016/0003-9861(54)90060-0. [DOI] [PubMed] [Google Scholar]
- Mill P. J. The pectic enzymes of Aspergillus niger. A mercury-activated exopolygalacturonase. Biochem J. 1966 Jun;99(3):557–561. doi: 10.1042/bj0990557. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mill P. J. The pectic enzymes of Aspergillus niger. A second exopolygalacturonase. Biochem J. 1966 Jun;99(3):562–565. doi: 10.1042/bj0990562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PRESSEY R., ALLEN R. S. PAPER CHROMATOGRAPHY OF OLIGOGALACTURONIDES. J Chromatogr. 1964 Oct;16:248–251. doi: 10.1016/s0021-9673(01)82474-2. [DOI] [PubMed] [Google Scholar]