Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1973 Sep;52(3):278–282. doi: 10.1104/pp.52.3.278

Cyclic Nucleotide Phosphodiesterase Activity in Barley Seeds 1,2

Jozef Vandepeute a,3, Ray C Huffaker a, Robert Alvarez a,4
PMCID: PMC366485  PMID: 16658547

Abstract

Barley seeds (Hordeum vulgare L. cv. Himalaya) contain an enzymatic activity which catalyzes the hydrolysis of adenosine cyclic 3′: 5′-monophosphate and adenosine cyclic 2′: 3′-monophosphate. A large portion of the enzymatic activity is present in the dry seed, existing in both soluble and particulate form. Secretion of the soluble phosphodiesterase from embryoless seeds is enhanced by gibberellic acid and inhibited by abscisic acid, dinitrophenol, and cycloheximide. Attempts to isolate or detect a phosphodiesterase which specifically hydrolyzes adenosine cyclic 3′: 5′-monophosphate were unsuccessful. Inhibition experiments indicate that probably one enzyme is involved in the hydrolysis of both of these substrates.

Full text

PDF
278

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azhar S., Murti C. R. Effect of indole-3-acetic acid on the synthesis of cyclic 3'-5' adenosine phosphate by Bengal gram seeds. Biochem Biophys Res Commun. 1971 Apr 2;43(1):58–64. doi: 10.1016/s0006-291x(71)80085-2. [DOI] [PubMed] [Google Scholar]
  2. Brunngraber E. F., Chargaff E. Nucleoside phosphotransferase from carrot. Kinetic studies and exploration of active sites. J Biol Chem. 1970 Sep 25;245(18):4825–4831. [PubMed] [Google Scholar]
  3. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duffus C. M., Duffus J. H. A possible role for cyclic AMP in gibberellic acid triggered release of alpha-amylase in barley endosperm slices. Experientia. 1969 Jun 15;25(6):581–581. doi: 10.1007/BF01896521. [DOI] [PubMed] [Google Scholar]
  5. Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lin P. P., Varner J. E. Cyclic nucleotide phosphodiesterase in pea seedlings. Biochim Biophys Acta. 1972 Aug 28;276(2):454–474. doi: 10.1016/0005-2744(72)91007-8. [DOI] [PubMed] [Google Scholar]
  8. Pollard C. J. Influence of gibberellic acid on the incorporation of 8-14C adenine into adenosine 3',5'-cyclic phosphate in barley aleurone layers. Biochim Biophys Acta. 1970 Mar 24;201(3):511–512. doi: 10.1016/0304-4165(70)90176-5. [DOI] [PubMed] [Google Scholar]
  9. Pollard C. J. Rapid gibberellin responses and the action of adenosine 3',5'-monophosphate in aleurone layers. Biochim Biophys Acta. 1971 Dec 21;252(3):553–560. doi: 10.1016/0304-4165(71)90158-9. [DOI] [PubMed] [Google Scholar]
  10. Robison G. A., Butcher R. W., Sutherland E. W. Cyclic AMP. Annu Rev Biochem. 1968;37:149–174. doi: 10.1146/annurev.bi.37.070168.001053. [DOI] [PubMed] [Google Scholar]
  11. Salomon D., Mascarenhas J. P. Auxin-induced synthesis of cyclic 3', 5'-adenosine monophosphate in Avena coleoptiles. Life Sci II. 1971 Aug;10(15):879–885. doi: 10.1016/0024-3205(71)90200-1. [DOI] [PubMed] [Google Scholar]
  12. Thompson W. J., Appleman M. M. Multiple cyclic nucleotide phosphodiesterase activities from rat brain. Biochemistry. 1971 Jan 19;10(2):311–316. [PubMed] [Google Scholar]
  13. Varner J. E. Gibberellic Acid Controlled Synthesis of alpha-Amylase in Barley Endosperm. Plant Physiol. 1964 May;39(3):413–415. doi: 10.1104/pp.39.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wood H. N., Lin M. C., Braun A. C. The inhibition of plant and animal adenosine 3':5'-cyclic monophosphate phosphodiesterases by a cell-division-promoting substance from tissues of higher plant species. Proc Natl Acad Sci U S A. 1972 Feb;69(2):403–406. doi: 10.1073/pnas.69.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES