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. Author manuscript; available in PMC: 2014 Mar 19.

Published in final edited form as: Biochemistry. 2013 Mar 8;52(11):1858–1873. doi: 10.1021/bi3015696

a. The dependence of the DnaT fluorescence anisotropy upon the total DnaT monomer concentration (λ_ex = 296 nm, λ_em = 347 nm) in buffer C (pH 7.0, 25°C) containing different concentrations of glycerol (w/v): 10 %, 15 %, 20 %, and 25 %. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model described by eqs. 3 - 7 (accompanying paper). b. The dependence of the DnaT fluorescence anisotropy upon the total DnaT monomer concentration (λ_ex = 296 nm, λ_em = 347 nm) in buffer C (pH 7.0, 25 °C) containing 0.1 mM EDTA and no magnesium, and different concentrations of glycerol (w/v): 10 %, 15 %, 20 %, and 25 %. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model described by eqs. 3 - 7 (accompanying paper). c. The dependences of the trimerization constant, K_T, upon the water concentration in the sample in the presence and absence of magnesium, respectively. The solid lines are linear least-squares fits with the slopes: ∂logK_T/∂log[H₂O] = 0 in the presence of Mg²⁺ and ∂logK_T/∂log[H₂O] = 11.1 in the absence of Mg²⁺, respectively (details in text).

Inline graphic — a. The dependence of the DnaT fluorescence anisotropy upon the total DnaT monomer concentration (λ_ex = 296 nm, λ_em = 347 nm) in buffer C (pH 7.0, 25°C) containing different concentrations of glycerol (w/v): 10 %, 15 %, 20 %, and 25 %. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model described by eqs. 3 - 7 (accompanying paper). b. The dependence of the DnaT fluorescence anisotropy upon the total DnaT monomer concentration (λ_ex = 296 nm, λ_em = 347 nm) in buffer C (pH 7.0, 25 °C) containing 0.1 mM EDTA and no magnesium, and different concentrations of glycerol (w/v): 10 %, 15 %, 20 %, and 25 %. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model described by eqs. 3 - 7 (accompanying paper). c. The dependences of the trimerization constant, K_T, upon the water concentration in the sample in the presence and absence of magnesium, respectively. The solid lines are linear least-squares fits with the slopes: ∂logK_T/∂log[H₂O] = 0 in the presence of Mg²⁺ and ∂logK_T/∂log[H₂O] = 11.1 in the absence of Mg²⁺, respectively (details in text).