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. 1973 Dec;52(6):633–637. doi: 10.1104/pp.52.6.633

Amino Acid Uptake by Pea Leaf Fragments

Specificity, Energy Sources, and Mechanism 1

Yuk-Ngai Stephen Cheung a, Park S Nobel a
PMCID: PMC366561  PMID: 16658620

Abstract

Amino acid uptake into leaf fragments of Pisum sativum depended on metabolism. Glycine uptake was optimal at 30 C and could be supported by respiration and by photosynthesis. Based on studies with an electron flow cofactor, inhibitors, and uncouplers, the energy source for glycine uptake was apparently ATP.

The energy-dependent transport of glycine was mediated by a carrier that had a broad specificity for neutral and positively charged l-amino acids. It readily translocated 15 such l-amino acids into the cells, but had a very low affinity for l-aspartate, l-glutamate, d-amino acids, and α-aminoisobutyrate. The Ki for competitive inhibition of glycine uptake by another amino acid was equal to the Km for the uptake of that competing species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Francki R. I., Zaitlin M., Jensen R. G. Metabolism of Separated Leaf Cells: II. Uptake and Incorporation of Protein and Ribonucleic Acid Precursors by Tobacco Cells. Plant Physiol. 1971 Jul;48(1):14–18. doi: 10.1104/pp.48.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Heldt H. W., Sauer F. The inner membrane of the chloroplast envelope as the site of specific metabolite transport. Biochim Biophys Acta. 1971 Apr 6;234(1):83–91. doi: 10.1016/0005-2728(71)90133-2. [DOI] [PubMed] [Google Scholar]
  3. Nobel P. S., Cheung Y. S. Two amino-acid carriers in pea chloroplasts. Nat New Biol. 1972 Jun 14;237(76):207–208. doi: 10.1038/newbio237207a0. [DOI] [PubMed] [Google Scholar]
  4. Nobel P. S. Relation of the Light-dependent Potassium Uptake by Pea Leaf Fragments to the pK of the Accompanying Organic Acid. Plant Physiol. 1970 Sep;46(3):491–493. doi: 10.1104/pp.46.3.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Osmond C. B., Harris B. Photorespiration during C 4 photosynthesis. Biochim Biophys Acta. 1971 May 11;234(2):270–282. doi: 10.1016/0005-2728(71)90082-x. [DOI] [PubMed] [Google Scholar]
  6. Smith R. C., Epstein E. Ion Absorption by Shoot Tissue: Technique and First Findings with Excised Leaf Tissue of Corn. Plant Physiol. 1964 May;39(3):338–341. doi: 10.1104/pp.39.3.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Stewart C. R. Effects of proline and carbohydrates on the metabolism of exogenous proline by excised bean leaves in the dark. Plant Physiol. 1972 Nov;50(5):551–555. doi: 10.1104/pp.50.5.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stewart C. R. The effect of wilting on proline metabolism in excised bean leaves in the dark. Plant Physiol. 1973 Mar;51(3):508–511. doi: 10.1104/pp.51.3.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Streeter J. G., Thompson J. F. Anaerobic Accumulation of gamma-Aminobutyric Acid and Alanine in Radish Leaves (Raphanus sativus, L.). Plant Physiol. 1972 Apr;49(4):572–578. doi: 10.1104/pp.49.4.572. [DOI] [PMC free article] [PubMed] [Google Scholar]

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