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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Gastroenterology. 2013 Feb 27;144(7):1543–1553.e1. doi: 10.1053/j.gastro.2013.02.037

Figure 3. Mst1 and Mst2 are required for postnatal maintenance of the exocrine pancreas.

Figure 3

(A) Total pancreas mass of DKO animal was compared to that of littermate controls at P0, P7, P14, and P30 (N=7 per group). A significant difference of body weight was observed on P30 (p=0.0013).

(B) Western blot assay of pancreas lysates at different time points, starting from E15 through P14, was performed using antibodies against YAP, and β−actin. 30 µg of pancreas lysate was loaded per well..

(C) qPCR was performed for Mst1, Mst2, and YAP at each of 4 time-points comparing control and DKO pancreata (n=3 animals in each group per time point). (mean +/− SD; *, P<0.05; **, P<0.01; ***, P<0.001).

(D) qPCR for the acinar transcription factors Mist1, Ptf1a, RBP-JL, and Lrh-1 were performed at P0 comparing control and DKO pancreata (n=3 animals in each group per time point).

(E) Pancreas tissues of DKO and littermate control were examined by H&E staining (i–viii), immunofluorescence for CK19 and CPA1 (ix–xvi), YAP and PDX1 (xvii,xxi) or YAP and E-Cadherin (xviii–xx, xxii–xxiv) at the indicated developmental stage. Note the strong nuclear YAP staining in the “trunk” regions of both control and DKO embryos at E15.5 and in the duct-like structures of DKO animals at P7 and P14. Scale bar: 100µM.