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. 1974 Nov;54(5):737–743. doi: 10.1104/pp.54.5.737

The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid

A Double Labeling Determination 1

Graham C Walker a, Nelson J Leonard a, Donald J Armstrong b, Norimoto Murai b, Folke Skoog b
PMCID: PMC366592  PMID: 16658962

Abstract

Cytokinin-dependent tobacco (Nicotiana tabacum) tissue was grown in the presence of N6-benzyladenine (6-benzylaminopurine) labeled in the phenyl ring with 3H and in the 8-position of the purine ring with 14C. The 3H/14C ratio of N6-benzyladenosine recovered from labeled tobacco callus transfer RNA preparations was compared with the corresponding ratio for N6-benzyladenine supplied in the medium. By this means, with suitable controls, the incorporation of the synthetic cytokinin, N6-benzyladenine, into the transfer RNA of tobacco callus tissue was shown to involve the intact moiety. The observed level of incorporation was up to one N6-benzyladenosine molecule per 104 transfer RNA molecules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. J., Burrows W. J., Evans P. K., Skoog F. Isolation of cytokinins from tRNA. Biochem Biophys Res Commun. 1969 Oct 22;37(3):451–456. doi: 10.1016/0006-291x(69)90936-x. [DOI] [PubMed] [Google Scholar]
  2. Bartz J. K., Söll D. N 6 -( 2 -isopentenyl) adenosine: biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli. Biochimie. 1972;54(1):31–39. doi: 10.1016/s0300-9084(72)80035-x. [DOI] [PubMed] [Google Scholar]
  3. Burrows W. J., Skoog F., Leonard N. J. Isolation and identification of cytokinins located in the transfer ribonucleic acid of tobacco callus grown in the presence of 6-benzylaminopurine. Biochemistry. 1971 Jun 8;10(12):2189–2194. doi: 10.1021/bi00788a002. [DOI] [PubMed] [Google Scholar]
  4. Deleuze G. G., McChesney J. D., Fox J. E. Identification of a stable cytokinin metabolite. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1426–1432. doi: 10.1016/0006-291x(72)90872-8. [DOI] [PubMed] [Google Scholar]
  5. Dyson W. H., Fox J. E., McChesney J. D. Short term metabolism of urea and purine cytokinins. Plant Physiol. 1972 Apr;49(4):506–513. doi: 10.1104/pp.49.4.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elliott D. C., Murray A. W. A quantitative limit for cytokinin incorporation into transfer ribonucleic acid by soya-bean callus tissue. Biochem J. 1972 Dec;130(4):1157–1160. doi: 10.1042/bj1301157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fittler F., Kline L. K., Hall R. H. N6-(Delta 2-isopentenyl)adenosine: biosynthesis in vitro by an enzyme extract from yeast and rat liver. Biochem Biophys Res Commun. 1968 May 23;31(4):571–576. doi: 10.1016/0006-291x(68)90516-0. [DOI] [PubMed] [Google Scholar]
  8. Fox J. E., Chen C. Characterization of labeled ribonucleic acid from tissue grown on 14C-containing cytokinins. J Biol Chem. 1967 Oct 10;242(19):4490–4494. [PubMed] [Google Scholar]
  9. Fox J. E. Incorporation of a kinin, N, 6-benzyladenine into soluble RNA. Plant Physiol. 1966 Jan;41(1):75–82. doi: 10.1104/pp.41.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fox J. E., Sood C. K., Buckwalter B., McChesney J. D. The metabolism and biological activity of a 9-substituted cytokinin. Plant Physiol. 1971 Feb;47(2):275–281. doi: 10.1104/pp.47.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kende H., Tavares J. E. On the significance of cytokinin incorporation into RNA. Plant Physiol. 1968 Aug;43(8):1244–1248. doi: 10.1104/pp.43.8.1244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kende H. The cytokinins. Int Rev Cytol. 1971;31:301–338. doi: 10.1016/s0074-7696(08)60061-3. [DOI] [PubMed] [Google Scholar]
  13. Kline L. K., Fittler F., Hall R. H. N6-(delta-2-isopentenyl) adenosine. Biosynthesis in transfer ribonucleic acid in vitro. Biochemistry. 1969 Nov;8(11):4361–4371. doi: 10.1021/bi00839a021. [DOI] [PubMed] [Google Scholar]
  14. Peterkofsky A. The incorporation of mevalonic acid into the N6-(delta 2-isopentenyl) adenosine of transfer ribonucleic acid in Lactobacillus acidophilus. Biochemistry. 1968 Jan;7(1):472–482. doi: 10.1021/bi00841a059. [DOI] [PubMed] [Google Scholar]
  15. Rosenbaum N., Gefter M. L. Delta 2 -isopentenylpyrophosphate: transfer ribonucleic acid 2 -isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme. J Biol Chem. 1972 Sep 25;247(18):5675–5680. [PubMed] [Google Scholar]
  16. Söll D. Enzymatic modification of transfer RNA. Science. 1971 Jul 23;173(3994):293–299. doi: 10.1126/science.173.3994.293. [DOI] [PubMed] [Google Scholar]
  17. Zachau H. G. Transfer ribonucleic acids. Angew Chem Int Ed Engl. 1969 Oct;8(10):711–727. doi: 10.1002/anie.196907111. [DOI] [PubMed] [Google Scholar]

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