Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1974 Nov;54(5):744–747. doi: 10.1104/pp.54.5.744

Phospholipids in the Developing Soybean Seed 1

Richard F Wilson a,2, Robert W Rinne a
PMCID: PMC366593  PMID: 16658963

Abstract

The distribution of phospholipids in developing soybean seeds [Glycine max (L.) Merr., var. “Chippewa 64,” “Harosoy 63,” “Wayne,” and “Clark 63”] was followed. From 30 to 60 days after flowering expressed as mole per cent of phospholipid phosphorus phosphatidic acid decreased from 14.8 to 9.1; phosphatidylinositol increased from 0 to 9.1; phosphatidylcholine increased from 8.2 to 9.8; phosphatidylethanolamine increased from 5.3 to 8.6; phosphatidylglycerol increased from 3.2 to 4.8; diphosphatidylglycerol increased from 2.7 to 4.1; and N-acylphosphatidylethanolamine decreased from 65.8 to 54.6. However, from 60 days after flowering to maturity, phosphatidic acid decreased to 0; phosphatidylinositol increased roughly 2-fold; phosphatidylcholine increased roughly 4.7-fold; phosphatidylethanolamine increased 3-fold; N-acylphosphatidylethanolamine decreased 11-fold; whereas phosphatidylglycerol and diphosphatidylglycerol remained essentially constant. Percentages of individual phospholipid species were not statistically different between any two varieties at a given time period.

Immature soybean cotyledons incubated with 14C-acetate or -pyruvate demonstrated rapid incorporation into the phospholipid fraction. N-acylphosphatidylethanolamine was found to account for nearly 70% of the total radioactivity incorporated by the total polar lipid fraction and greater than 30% of the total radioactivity added.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aneja R., Chadha J. S., Knaggs J. A. N-Acylphosphatidylethanolamines: occurrence in nature, structure and stereochemistry. Biochem Biophys Res Commun. 1969 Aug 7;36(3):401–406. doi: 10.1016/0006-291x(69)90578-6. [DOI] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. Bomstein R. A. A new class of phosphatides isolated from soft wheat flour. Biochem Biophys Res Commun. 1965 Oct 8;21(1):49–54. doi: 10.1016/0006-291x(65)90424-9. [DOI] [PubMed] [Google Scholar]
  4. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  5. Dawson R. M., Clarke N., Quarles R. H. N-acylphosphatidylethanolamine, a phospholipid that is rapidly metabolized during the arly germnation of pea seeds. Biochem J. 1969 Sep;114(2):265–267. doi: 10.1042/bj1140265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KENNEDY E. P. Biosynthesis of complex lipids. Fed Proc. 1961 Dec;20:934–940. [PubMed] [Google Scholar]
  7. Privett O. S., Dougherty K. A., Erdahl W. L., Stolyhwo A. Studies on the lipid composition of developing soybeans. J Am Oil Chem Soc. 1973 Dec;50(12):516–520. doi: 10.1007/BF02640523. [DOI] [PubMed] [Google Scholar]
  8. Singh H., Privett O. S. Incorporation of 33P in soybean phosphatides. Biochim Biophys Acta. 1970 Feb 10;202(1):200–202. doi: 10.1016/0005-2760(70)90236-5. [DOI] [PubMed] [Google Scholar]
  9. Singh H., Privett O. S. Studies on the glycolipids and phospholipids of immature soybeans. Lipids. 1970 Aug;5(8):692–697. doi: 10.1007/BF02531436. [DOI] [PubMed] [Google Scholar]
  10. de la Roche I. A., Andrews C. J. Changes in Phospholipid Composition of a Winter Wheat Cultivar during Germination at 2 C and 24 C. Plant Physiol. 1973 Mar;51(3):468–473. doi: 10.1104/pp.51.3.468. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES