Abstract
Urease and ribulose 1, 5-diphosphate carboxylase can be bound to Sepharose to give an immobilized two-enzyme system which catalyzes the reaction urea → H2CO3 → phosphoglyceric acid. The observed Km of the system for urea approaches the lower value for urease when carboxylase levels on the gel exceed urease levels. If a similar system operates in the chloroplast, the high Km (H2CO3) of ribulose 1,5-diphosphate carboxylase may not be metabolically significant.
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Selected References
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- Anderson L. E., Fuller R. C. Photosynthesis in Rhodospirillum rubrum. IV. Isolation and characterization of ribulose 1,5-diphosphate carboxylase. J Biol Chem. 1969 Jun 25;244(12):3105–3109. [PubMed] [Google Scholar]
- Bahr J. T., Jensen R. G. Ribulose Diphosphate Carboxylase from Freshly Ruptured Spinach Chloroplasts Having an in Vivo Km[CO(2)]. Plant Physiol. 1974 Jan;53(1):39–44. doi: 10.1104/pp.53.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooper T. G., Filmer D. The active species of "CO2" utilized by ribulose diphosphate carboxylase. J Biol Chem. 1969 Feb 10;244(3):1081–1083. [PubMed] [Google Scholar]
- Gestrelius S., Mattiasson B., Mosbach K. On the regulation of the activity of immobilized enzymes. Microenvironmental effects of enzyme-generated pH changes. Eur J Biochem. 1973 Jul 2;36(1):89–96. doi: 10.1111/j.1432-1033.1973.tb02888.x. [DOI] [PubMed] [Google Scholar]
- Hamilton B. K., Stockmeyer L. J., Colton C. K. Comments on diffusive and electrostatic effects with immobilized enzymes. J Theor Biol. 1973 Oct;41(3):547–560. doi: 10.1016/0022-5193(73)90061-1. [DOI] [PubMed] [Google Scholar]
- Hanson K. R., Ling R., Havir E. A computer program for fitting data to the Michaelis-Menten equation. Biochem Biophys Res Commun. 1967 Oct 26;29(2):194–197. doi: 10.1016/0006-291x(67)90586-4. [DOI] [PubMed] [Google Scholar]
- Jensen R. G., Bassham J. A. Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095–1101. doi: 10.1073/pnas.56.4.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paulsen J. M., Lane M. D. Spinach ribulose diphosphate carboxylase. I. Purification and properties of the enzyme. Biochemistry. 1966 Jul;5(7):2350–2357. doi: 10.1021/bi00871a025. [DOI] [PubMed] [Google Scholar]
- Srere P. A., Mattiasson B., Mosbach K. An immobilized three-enzyme system: a model for microenvironmental compartmentation in mitochondria. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2534–2538. doi: 10.1073/pnas.70.9.2534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sundaram P. V. The kinetic properties of microencapsulated urease. Biochim Biophys Acta. 1973 Sep 15;321(1):319–328. doi: 10.1016/0005-2744(73)90086-7. [DOI] [PubMed] [Google Scholar]
- Werdan K., Heldt H. W. Accumulation of bicarbonate in intact chloroplasts following a pH gradient. Biochim Biophys Acta. 1972 Dec 14;283(3):430–441. doi: 10.1016/0005-2728(72)90260-5. [DOI] [PubMed] [Google Scholar]
