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. 1979 Jan;137(1):409–414. doi: 10.1128/jb.137.1.409-414.1979

Phosphoglucose isomerase mutant of Rhizobium meliloti.

A Arias, C Cerveńansky, A Gardiol, G Martínez-Drets
PMCID: PMC366610  PMID: 762017

Abstract

A mutant strain of complex phenotype was selected in Rhizobium meliloti after nitrosoguanidine mutagenesis. It failed to grow on mannitol, sorbitol, fructose, mannose, ribose, arabitol, or xylose, but grew on glucose, maltose, gluconate, L-arabinose, and many other carbohydrates. Assay showed the enzyme lesion to be in phosphoglucose isomerase (pgi), and revertants, which were of normal growth phenotype, contained the enzyme again. Nonpermissive substrates such as fructose and xylose prevented growth on permissive ones such as L-arabinose, and in such situations there was high accumulation of fructose 6-phosphate. The mutant strain had about 20% as much exopolysaccharide as the parent. Nitrogen fixation by whole plants was low and delayed when the mutant strain was the inoculant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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