Abstract
Cell-free extracts from gibberellic acid-treated barley (Hordeum vulgare L. cv. Himalaya) aleurone layers show phosphorylcholine glyceride transferase activity greater than that from control layers. The increase in activity is not prevented by a mixture of amino acid analogs nor by cordycepin under conditions in which it is demonstrated that the analogs and the cordycepin are entering the cells in effective concentrations. We conclude therefore that the GA3-dependent increase in phosphorylcholine glyceride transferase activity (which occurs within the first 4 hours of GA3 treatment) does not require RNA synthesis or protein synthesis.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bennett P. A., Chrispeels M. J. De Novo Synthesis of Ribonuclease and beta-1,3-Glucanase by Aleurone Cells of Barley. Plant Physiol. 1972 Mar;49(3):445–447. doi: 10.1104/pp.49.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chrispeels M. J. Mechanism of osmotic regulation of hydrolase synthesis in aleurone cells of barley: inhibition of protein synthesis. Biochem Biophys Res Commun. 1973 Jul 2;53(1):99–104. doi: 10.1016/0006-291x(73)91406-x. [DOI] [PubMed] [Google Scholar]
- Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellis R. J., Macdonald I. R. Specificity of cycloheximide in higher plant systems. Plant Physiol. 1970 Aug;46(2):227–232. doi: 10.1104/pp.46.2.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evins W. H., Varner J. E. Hormonal control of polyribosome formation in barley aleurone layers. Plant Physiol. 1972 Mar;49(3):348–352. doi: 10.1104/pp.49.3.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferrari T. E., Varner J. E. Control of nitrate reductase activity in barley aleurone layers. Proc Natl Acad Sci U S A. 1970 Mar;65(3):729–736. doi: 10.1073/pnas.65.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferrari T. E., Varner J. E. Substrate induction of nitrate reductase in barley aleurone layers. Plant Physiol. 1969 Jan;44(1):85–88. doi: 10.1104/pp.44.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Filner P., Varner J. E. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520–1526. doi: 10.1073/pnas.58.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honig G. R., Rabinovitz M. Actinomycin D: inhibition of protein synthesis unrelated to effect on template RNA synthesis. Science. 1965 Sep 24;149(3691):1504–1506. doi: 10.1126/science.149.3691.1504. [DOI] [PubMed] [Google Scholar]
- Jacobsen J. V., Varner J. E. Gibberellic Acid-induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol. 1967 Nov;42(11):1596–1600. doi: 10.1104/pp.42.11.1596. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson K. D., Kende H. Hormonal Control of Lecithin Synthesis in Barley Aleurone Cells: Regulation of the CDP-Choline Pathway by Gibberellin. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2674–2677. doi: 10.1073/pnas.68.11.2674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koehler D. E., Varner J. E. Hormonal control of orthophosphate incorporation into phospholipids of barley aleurone layers. Plant Physiol. 1973 Sep;52(3):208–214. doi: 10.1104/pp.52.3.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laszlo J., Miller D. S., McCarty K. S., Hochstein P. Actinomycin D: inhibition of respiration and glycolysis. Science. 1966 Feb 25;151(3713):1007–1010. doi: 10.1126/science.151.3713.1007. [DOI] [PubMed] [Google Scholar]
- Ross C. Influence of cycloheximide (Actidione) upon pyrimidine nucleotide metabolism and rna synthesis in cocklebur leaf discs. Biochim Biophys Acta. 1968 Aug 23;166(1):40–47. doi: 10.1016/0005-2787(68)90488-7. [DOI] [PubMed] [Google Scholar]
- Schwartz R. J. Control of glutamine synthetase synthesis in the embryonic chick neural retina. A caution in the use of actinomycin D. J Biol Chem. 1973 Sep 25;248(18):6426–6435. [PubMed] [Google Scholar]