Table 2.
Average values for final fits to extended model-free using τc= 17.7 ns for the conjugate and 21.8 ns for ternary complexes. Averages are based on all residues (#aa) for which reasonable values were fit (ie S2 not 0 or 1) using all data available. All of the final analyses were based on the results obtained for the E4B mutant; the results with WT E4B are included for comparison.
| E2 | Ub | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| #aa | S2 | S2f | S2s | τs ns | #aa | S2 | S2f | S2s | τs ns | |
| UbcH5c-O-Ub | 58 | 0.89 +/− 0.05 |
0.95 +/− 0.04 |
0.92 +/− 0.03 |
0.71 +/− 1.31 |
31 | 0.65 +/− 0.03 |
0.83 +/− 0.03 |
0.78 +/− 0.03 |
3.2 +/− 0.72 |
|
UbcH5c-O-Ub/
E4BU R1143A |
41 | 0.85 +/− 0.07 |
0.89 +/− 0.06 |
0.92 +/− 0.06 |
0.49 +/− 0.72 |
46 | 0.79 +/− 0.03 |
0.92 +/− 0.03 |
0.85 +/− 0.02 |
1.7 +/− 0.23 |
|
UbcH5c-0-Ub/
WT E4BU |
26 |
0.80 +/−
0.25 |
0.88 +/−
0.21 |
0.91 +/−
0.23 |
0.49 +/−
1.19 |
24 |
0.85
+/− 0.30 |
0.95 +/−
0.23 |
0.85 +/−
0.28 |
2.4 +/−
1.47 |