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. 1985 Mar;5(3):466–473. doi: 10.1128/mcb.5.3.466

Malignant transformation and tumor promoter treatment increase levels of a transcript for a secreted glycoprotein.

P J Doherty, L Hua, G Liau, S Gal, D E Graham, M Sobel, M M Gottesman
PMCID: PMC366738  PMID: 3990681

Abstract

The major excreted protein of transformed mouse fibroblasts, a secreted, mannose 6-phosphate-containing glycoprotein, is induced in nontransformed cells by a variety of transforming agents, by phorbol esters, and by platelet-derived growth factor. We report here the molecular cloning of the cDNA encoding this protein and demonstrate that its induction is a consequence of enhanced mRNA levels for major excreted protein in both tetradecanoyl phorbol acetate-treated 3T3 cells and 3T3 cells transformed by a variety of retroviruses or retroviral oncogenes. These results indicate that tumor promoters and retroviral transformation might share a common pathway of action in cultured cells and that major excreted protein is a molecular marker for the growth response of cells to these agents.

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