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. 1985 Apr;5(4):728–733. doi: 10.1128/mcb.5.4.728

Cells transformed with a ts viral src mutant are temperature sensitive for in vivo growth.

A F Chambers, S Wilson
PMCID: PMC366776  PMID: 3921824

Abstract

Studies on ts mutants of avian sarcoma viruses have previously implicated the src gene product (pp60src) kinase function in in vitro transformation. The role of src in vivo, however, has not been clearly defined. Using a sensitive and quantitative assay that was developed in chicken embryos (Chambers et al., Cancer Res. 42:4018-4025, 1982), we tested the in vivo tumorigenic properties of cells transformed with LA23, an avian sarcoma virus that is temperature sensitive for in vitro transformation. We found that the in vivo growth ability of these cells was temperature sensitive and that this in vivo behavior correlated with the in vitro transformation behavior (growth in soft agar and saturation density).

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Selected References

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  1. Armstrong P. B., Quigley J. P., Sidebottom E. Transepithelial invasion and intramesenchymal infiltration of the chick embryo chorioallantois by tumor cell lines. Cancer Res. 1982 May;42(5):1826–1837. [PubMed] [Google Scholar]
  2. Becker D., Kurth R., Critchley D., Friis R., Bauer H. Distinguishable transformation-defective phenotypes among temperature-sensitive mutants of Rous sarcoma virus. J Virol. 1977 Mar;21(3):1042–1055. doi: 10.1128/jvi.21.3.1042-1055.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop J. M. Cellular oncogenes and retroviruses. Annu Rev Biochem. 1983;52:301–354. doi: 10.1146/annurev.bi.52.070183.001505. [DOI] [PubMed] [Google Scholar]
  4. Chambers A. F., Ling V. Selection for experimental metastatic ability of heterologous tumor cells in the chick embryo after DNA-mediated transfer. Cancer Res. 1984 Sep;44(9):3970–3975. [PubMed] [Google Scholar]
  5. Chambers A. F., Shafir R., Ling V. A model system for studying metastasis using the embryonic chick. Cancer Res. 1982 Oct;42(10):4018–4025. [PubMed] [Google Scholar]
  6. Chen Y. C., Hayman M. J., Vogt P. K. Properties of mammalian cells transformed by temperature-sensitive mutants of avian sarcoma virus. Cell. 1977 Jul;11(3):513–521. doi: 10.1016/0092-8674(77)90069-1. [DOI] [PubMed] [Google Scholar]
  7. Collett M. S., Erikson E., Erikson R. L. Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation. J Virol. 1979 Feb;29(2):770–781. doi: 10.1128/jvi.29.2.770-781.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cooper G. M. Cellular transforming genes. Science. 1982 Aug 27;217(4562):801–806. doi: 10.1126/science.6285471. [DOI] [PubMed] [Google Scholar]
  9. Darlix J. L., Bromley P. A., Spahr P. F. Characterization of the src gene of a Rous sarcoma virus mutant temperature-sensitive for cell transformation. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):843–847. doi: 10.1101/sqb.1980.044.01.089. [DOI] [PubMed] [Google Scholar]
  10. Dolberg D. S., Bissell M. J. Inability of Rous sarcoma virus to cause sarcomas in the avian embryo. Nature. 1984 Jun 7;309(5968):552–556. doi: 10.1038/309552a0. [DOI] [PubMed] [Google Scholar]
  11. Durkin J. P., Boynton A. L., Whitfield J. F. The src gene product (pp60 src) of avian sarcoma virus rapidly induces DNA synthesis and proliferation of calcium-deprived rat cells. Biochem Biophys Res Commun. 1981 Nov 16;103(1):233–239. doi: 10.1016/0006-291x(81)91684-3. [DOI] [PubMed] [Google Scholar]
  12. Durkin J. P., Whitfield J. F., MacManus J. P. The role of calmodulin in the proliferation of transformed and phenotypically normal tsASV-infected rat cells. J Cell Physiol. 1983 Jun;115(3):313–319. doi: 10.1002/jcp.1041150315. [DOI] [PubMed] [Google Scholar]
  13. EBERT J. D., WILT F. H. Animal viruses and embryos. Q Rev Biol. 1960 Dec;35:261–312. doi: 10.1086/403189. [DOI] [PubMed] [Google Scholar]
  14. Easty G. C., Easty D. M., Tchao R. The growth of heterologous tumour cells in chick embryos. Eur J Cancer. 1969 Jun;5(3):287–295. doi: 10.1016/0014-2964(69)90079-6. [DOI] [PubMed] [Google Scholar]
  15. Friis R. R., Jockusch B. M., Boschek C. B., Ziemiecki A., Rübsamen H., Bauer H. Transformation-defective, temperature-sensitive mutants of Rous sarcoma virus have a reversibly defective src-gene product. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):1007–1012. doi: 10.1101/sqb.1980.044.01.108. [DOI] [PubMed] [Google Scholar]
  16. Friis R. R. Temperature-sensitive mutants of avian RNA tumor viruses: a review. Curr Top Microbiol Immunol. 1978;79:261–293. doi: 10.1007/978-3-642-66853-1_6. [DOI] [PubMed] [Google Scholar]
  17. Fung Y. K., Crittenden L. B., Fadly A. M., Kung H. J. Tumor induction by direct injection of cloned v-src DNA into chickens. Proc Natl Acad Sci U S A. 1983 Jan;80(2):353–357. doi: 10.1073/pnas.80.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. GREEN H., LORINCZ A. L. The role of a natural antibody in the rejection of mouse tumor cells by the chick embryo. J Exp Med. 1957 Jul 1;106(1):111–126. doi: 10.1084/jem.106.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Graf T., Friis R. R. Differential expression of transformation in rat and chicken cells infected with an avian sarcoma virus ts mutant. Virology. 1973 Nov;56(1):369–374. doi: 10.1016/0042-6822(73)90314-0. [DOI] [PubMed] [Google Scholar]
  20. Huu Duc-Nguyen, Rosenblum E. N., Zeigel R. F. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J Bacteriol. 1966 Oct;92(4):1133–1140. doi: 10.1128/jb.92.4.1133-1140.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. KARNOFSKY D. A., RIDGWAY L. P., PATTERSON P. A. Tumor transplantation to the chick embryo. Ann N Y Acad Sci. 1952 Aug 8;55(2):313–329. doi: 10.1111/j.1749-6632.1952.tb26547.x. [DOI] [PubMed] [Google Scholar]
  22. Kawai S., Hanafusa H. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant. Virology. 1971 Nov;46(2):470–479. doi: 10.1016/0042-6822(71)90047-x. [DOI] [PubMed] [Google Scholar]
  23. Klarlund J. K., Forchhammer J. Temperature-sensitive tumorigenicity of cells transformed by a mutant of Moloney sarcoma virus. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1501–1505. doi: 10.1073/pnas.77.3.1501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LEIGHTON J. INVASION AND METASTASIS OF HETEROLOGOUS TUMORS IN THE CHICK EMBRYO. Prog Exp Tumor Res. 1964;4:98–125. doi: 10.1159/000385975. [DOI] [PubMed] [Google Scholar]
  25. Lehman J. M., Estes P. Tumorigenicity of tsA and wild-type simian virus 40 transformed cells inoculated onto the chicken chorioallantoic membrane. Int J Cancer. 1982 Nov 15;30(5):643–648. doi: 10.1002/ijc.2910300515. [DOI] [PubMed] [Google Scholar]
  26. METCALF D. Pseudoneoplastic nonspecific proliferative lesions on the chorio-allantois of the chick embryo. Br J Cancer. 1955 Mar;9(1):222–228. doi: 10.1038/bjc.1955.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Owada M., Moelling K. Temperature-sensitive kinase activity associated with various mutants of avian sarcoma viruses which are temperature sensitive for transformation. Virology. 1980 Feb;101(1):157–168. doi: 10.1016/0042-6822(80)90492-4. [DOI] [PubMed] [Google Scholar]
  28. Poirier F., Lawrence D., Vigier P., Jullien P. A ts T mutant of Schmidt Ruppin strain of Rous sarcoma virus restricted at 39.5 degrees C for the morphological transformation and the tumorigenicity of chicken embryo fibroblasts. Int J Cancer. 1982 Jan 15;29(1):69–76. doi: 10.1002/ijc.2910290112. [DOI] [PubMed] [Google Scholar]
  29. Poste G., Flood M. K. Cells transformed by temperature-sensitive mutants of avian sarcoma virus cause tumors in vivo at permissive and nonpermissive temperatures. Cell. 1979 Aug;17(4):789–800. doi: 10.1016/0092-8674(79)90319-2. [DOI] [PubMed] [Google Scholar]
  30. Sorge L. K., Levy B. T., Maness P. F. pp60c-src is developmentally regulated in the neural retina. Cell. 1984 Feb;36(2):249–257. doi: 10.1016/0092-8674(84)90218-6. [DOI] [PubMed] [Google Scholar]
  31. Tickle C., Crawley A., Roscoe J. P. Survival of cells implanted in the embryonic chick limb bud: a difference between normal and malignant rat brain cells. J Cell Sci. 1979 Jun;37:143–156. doi: 10.1242/jcs.37.1.143. [DOI] [PubMed] [Google Scholar]
  32. Toyoshima K., Owada M., Kozai Y. Tumor producing capacity of temperature sensitive mutants of avian sarcoma viruses in chicks. Biken J. 1973 Sep;16(3):103–110. [PubMed] [Google Scholar]
  33. Weinberg R. A. Oncogenes of spontaneous and chemically induced tumors. Adv Cancer Res. 1982;36:149–163. doi: 10.1016/s0065-230x(08)60424-8. [DOI] [PubMed] [Google Scholar]
  34. Wyke J. A., Linial M. Temperature-sensitive avian sarcoma viruses: a physiological comparison of twenty mutants. Virology. 1973 May;53(1):152–161. doi: 10.1016/0042-6822(73)90474-1. [DOI] [PubMed] [Google Scholar]
  35. Wyke J. A. Temperature sensitive mutants of avian sarcoma viruses. Biochim Biophys Acta. 1975 Jul 11;417(2):91–121. doi: 10.1016/0304-419x(75)90001-3. [DOI] [PubMed] [Google Scholar]
  36. Wyke J. A. The selective isolation of temperature-sensitive mutants of Rous sarcoma virus. Virology. 1973 Apr;52(2):587–590. doi: 10.1016/0042-6822(73)90357-7. [DOI] [PubMed] [Google Scholar]

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