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. 1985 Apr;5(4):742–750. doi: 10.1128/mcb.5.4.742

Two integrated partial repeats of simian virus 40 together code for a super-T antigen.

A Levitt, S Chen, G Blanck, D George, R E Pollack
PMCID: PMC366778  PMID: 2985968

Abstract

We determined that the coding sequence for a 100-kilodalton super-T antigen found in Simian virus 40 mouse transformants spanned two separate partial repeats of the viral genome. The downstream repeat contained a complete Simian virus 40 large-T-antigen gene, whereas the upstream repeat was a truncated copy of the same gene. When the repeats were separated by subcloning, the capacity to code for the super-T antigen was lost. A small insertion or deletion in the origin-control region which preceded the second repeat could also destroy the ability to code for the 100-kilodalton protein. Our data suggest that differential splicing between parts of two gene copies was responsible for the additional molecular weight of this super-T antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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