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. 1985 Apr;5(4):898–901. doi: 10.1128/mcb.5.4.898

Drosophila P element-enhanced transfection in mammalian cells.

D W Clough, H M Lepinske, R L Davidson, R V Storti
PMCID: PMC366797  PMID: 2985975

Abstract

We constructed a gene transfer vector containing the herpes simplex virus type 1 thymidine kinase (TK) gene flanked by Drosophila P element terminal repeats (W. R. Engels, Annu. Rev. Genet. 17:315-344). This vector was introduced into mouse LTK- cells and enhanced the frequency of stable transformation to the TK+ phenotype by approximately 50-fold relative to a similar plasmid lacking the P element terminal repeats.

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Selected References

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