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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Bone. 2013 Apr 4;55(1):78–83. doi: 10.1016/j.bone.2013.03.014

Figure 5. Cartilage to bone conversion in the C2 vertebral epiphysis.

Figure 5

Mid-sagittal histological sections double-stained with Alcian blue (glycosaminoglycans) and picrosirius red (collagen) illustrating increased cartilage in the ventral epiphyses (*) at 3 months-of-age (A and B) and at 6 months-of-age (C and D). At 12 months-of age, the epiphyseal growth plate in normal animals was absent (E); however, in MPS I animals, this growth plate persisted (F). Scale bars = 500 μm (A–D) and 1 mm (E and F); gp = growth plate and ep = caudal vertebral endplate. All images are oriented with the ventral side at the bottom.