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. 1985 Jun;5(6):1531–1533. doi: 10.1128/mcb.5.6.1531

Induction of cellular DNA synthesis by a simian virus 40 mutant defective in nuclear transport of T antigen.

R E Lanford, J K Hyland, R Baserga, J S Butel
PMCID: PMC366887  PMID: 2993869

Abstract

The simian virus 40 (SV40) (cT)-3 mutant [SV40(cT)-3], which is defective in nuclear transport of T antigen, was utilized to determine whether cellular DNA synthesis can be stimulated by SV40 in the absence of detectable nuclear T antigen. Cellular DNA synthesis was examined in the temperature-sensitive cell cycle mutants, BHK ts13 and BHK tsAF8, after microinjection of quiescent cells with plasmid DNA containing cloned copies of wild-type SV40 or SV40(cT)-3. The efficiency of induction of cellular DNA synthesis was identical for both wild-type SV40 and SV40(cT)-3 in both cell lines. The results suggest that cell surface-associated T antigen, either alone or possibly in combination with minimal amounts of nuclear T antigen below our limit of detection, is able to stimulate cellular DNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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