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Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1985 Aug;5(8):1993–1996. doi: 10.1128/mcb.5.8.1993

Nuclear polyadenylate-binding protein.

A B Sachs, R D Kornberg
PMCID: PMC366917  PMID: 3837854

Abstract

Polyadenylate-binding activity can be detected in eluates from sodium dodecyl sulfate gels by a nitrocellulose filter-binding assay. Nuclear extracts from rat liver show a single peak of binding activity at 50 to 55 kilodaltons; cytoplasmic extracts show a single peak at 70 to 80 kilodaltons, corresponding to a 75-kilodalton protein previously described. Similar results are obtained with yeast and mouse fibroblasts, indicating a high degree of conservation of both nuclear and cytoplasmic polyadenylate-binding proteins. The activity from rat liver nuclei has been purified 125-fold on the basis of specific binding to polyadenylate and shows two main bands in sodium dodecyl sulfate gels at 53 and 55 kilodaltons.

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1993

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Selected References

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