Skip to main content
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1985 Aug;5(8):2147–2149. doi: 10.1128/mcb.5.8.2147

Human glyceraldehyde-3-phosphate dehydrogenase: mRNA levels and enzyme activity in developing muscle.

Y H Edwards, J C Lloyd, S L McMillan, F J Benham
PMCID: PMC366933  PMID: 3837856

Abstract

Analysis of human glyceraldehyde-3-phosphate dehydrogenase mRNA revealed that levels in adult skeletal muscle are 12-fold greater per microgram of polyadenylated RNA than in fetal skeletal muscle, whereas in cardiac muscle RNA levels were about equal in fetal and adult tissue. The mRNA levels correlate well with glyceraldehyde 3-phosphate dehydrogenase enzyme activities. There was no evidence for fetus- or tissue-specific forms.

Full text

PDF
2147

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benham F. J., Hodgkinson S., Davies K. E. A glyceraldehyde-3-phosphate dehydrogenase pseudogene on the short arm of the human X chromosomes defines a multigene family. EMBO J. 1984 Nov;3(11):2635–2640. doi: 10.1002/j.1460-2075.1984.tb02186.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bruns G. A., Gerald P. S. Human glyceraldehyde-3-phosphate dehydrogenase in man-rodent somatic cell hybrids. Science. 1976 Apr 2;192(4234):54–56. doi: 10.1126/science.176725. [DOI] [PubMed] [Google Scholar]
  3. Charlesworth D. Starch-gel electrophoresis of four enzymes from human red blood cells: glyceraldehyde-3-phosphate dehydrogenase, fructoaldolase, glyoxalase II and sorbitol dehydrogenase. Ann Hum Genet. 1972 Apr;35(4):477–484. doi: 10.1111/j.1469-1809.1957.tb01873.x. [DOI] [PubMed] [Google Scholar]
  4. Devlin R. B., Emerson C. P., Jr Coordinate accumulation of contractile protein mRNAs during myoblast differentiation. Dev Biol. 1979 Mar;69(1):202–216. doi: 10.1016/0012-1606(79)90286-0. [DOI] [PubMed] [Google Scholar]
  5. Edwards Y. H., Clark P., Harris H. Isozymes of glyceraldehyde-3-phosphate dehydrogenase in man and other mammals. Ann Hum Genet. 1976 Jul;40(1):67–77. doi: 10.1111/j.1469-1809.1976.tb00165.x. [DOI] [PubMed] [Google Scholar]
  6. Gillespie G., Lloyd J., Hopkinson D., Edwards Y. The 3'-untranslated sequence of human skeletal muscle alpha-actin mRNA. J Muscle Res Cell Motil. 1984 Aug;5(4):457–464. doi: 10.1007/BF00818263. [DOI] [PubMed] [Google Scholar]
  7. Gunning P., Ponte P., Blau H., Kedes L. alpha-skeletal and alpha-cardiac actin genes are coexpressed in adult human skeletal muscle and heart. Mol Cell Biol. 1983 Nov;3(11):1985–1995. doi: 10.1128/mcb.3.11.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hanauer A., Mandel J. L. The glyceraldehyde 3 phosphate dehydrogenase gene family: structure of a human cDNA and of an X chromosome linked pseudogene; amazing complexity of the gene family in mouse. EMBO J. 1984 Nov;3(11):2627–2633. doi: 10.1002/j.1460-2075.1984.tb02185.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  10. Minty A. J., Caravatti M., Robert B., Cohen A., Daubas P., Weydert A., Gros F., Buckingham M. E. Mouse actin messenger RNAs. Construction and characterization of a recombinant plasmid molecule containing a complementary DNA transcript of mouse alpha-actin mRNA. J Biol Chem. 1981 Jan 25;256(2):1008–1014. [PubMed] [Google Scholar]
  11. Scopes R. K. Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species. Biochem J. 1968 Mar;107(2):139–150. doi: 10.1042/bj1070139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shani M., Zevin-Sonkin D., Saxel O., Carmon Y., Katcoff D., Nudel U., Yaffe D. The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of corresponding mRNA sequences during myogenesis. Dev Biol. 1981 Sep;86(2):483–492. doi: 10.1016/0012-1606(81)90206-2. [DOI] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES