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. 1985 Oct;5(10):2842–2846. doi: 10.1128/mcb.5.10.2842

Surprising S1-resistant trimolecular hybrids: potential complication in interpretation of S1 mapping analyses.

M A Lopata, B Sollner-Webb, D W Cleveland
PMCID: PMC367024  PMID: 3016519

Abstract

Although the technique of S1 mapping is a powerful analytical tool for the analysis of RNA, we now report a surprising complication involving a trimolecular hybrid between two RNA species and a single DNA probe molecule which, if unrecognized, can lead to misleading interpretations. We document that such trimolecular hybrids can be efficiently formed under some hybridization conditions and that the probe DNA sequence at the junction of the two RNA molecules can be remarkably stable to digestion with S1. Trimolecular hybrids can arise in any instance whenever a distal region of an end-labeled DNA probe is homologous to a moderately abundant RNA in the sample to be analyzed. This situation presents a serious, potential complication for a variety of S1 analyses, particularly those in which DNA transfection has been utilized to reintroduce in vitro-engineered genes into cultured animal cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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