AT1R (A) and Nox2 (B) protein levels were determined by Western blot, respectively, in aortas isolated from sham, ovariectomy (OVX), and OVX + E2 groups of female offspring that had been exposed in utero to saline control or nicotine. The protein density was normalized to β-actin, and then expressed as to the relative value of the saline control. All of the data are expressed as means ± SEM of tissues from 4 animals in each group. *P<0.05, nicotine vs. saline control.