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. 1986 Oct;6(10):3368–3372. doi: 10.1128/mcb.6.10.3368

Anaerobic treatment of maize roots affects transcription of Adh1 and transcript stability.

L J Rowland, J N Strommer
PMCID: PMC367082  PMID: 3796583

Abstract

Among the adaptations to stress exhibited by plants is the anaerobic response of roots, induced by submerging roots in water. The response consists of a programmed change in gene expression: proteins produced under aerobic conditions are no longer synthesized but are replaced by approximately 20 so-called anaerobic peptides (M. M. Sachs, M. Freeling, and R. Okimoto, Cell 20:761-767, 1980). The gene for maize alcohol dehydrogenase I (Adh1) is expressed at high levels under such conditions. We report here that changes in alcohol dehydrogenase I RNA levels in anaerobic roots are associated with changes in both transcription rate and transcript stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Stark G. R., Wahl G. M. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. doi: 10.1016/0076-6879(79)68017-5. [DOI] [PubMed] [Google Scholar]
  2. Ashburner M., Bonner J. J. The induction of gene activity in drosophilia by heat shock. Cell. 1979 Jun;17(2):241–254. doi: 10.1016/0092-8674(79)90150-8. [DOI] [PubMed] [Google Scholar]
  3. Bennetzen J. L., Swanson J., Taylor W. C., Freeling M. DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4125–4128. doi: 10.1073/pnas.81.13.4125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  5. Bruskin A. M., Tyner A. L., Wells D. E., Showman R. M., Klein W. H. Accumulation in embryogenesis of five mRNAs enriched in the ectoderm of the sea urchin pluteus. Dev Biol. 1981 Oct 30;87(2):308–318. doi: 10.1016/0012-1606(81)90154-8. [DOI] [PubMed] [Google Scholar]
  6. Dennis E. S., Gerlach W. L., Pryor A. J., Bennetzen J. L., Inglis A., Llewellyn D., Sachs M. M., Ferl R. J., Peacock W. J. Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984 May 11;12(9):3983–4000. doi: 10.1093/nar/12.9.3983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DiDomenico B. J., Bugaisky G. E., Lindquist S. Heat shock and recovery are mediated by different translational mechanisms. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6181–6185. doi: 10.1073/pnas.79.20.6181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ferl R. J., Brennan M. D., Schwartz D. In vitro translation of maize ADH: evidence for the anaerobic induction of mRNA. Biochem Genet. 1980 Aug;18(7-8):681–691. doi: 10.1007/BF00484585. [DOI] [PubMed] [Google Scholar]
  9. Gerlach W. L., Pryor A. J., Dennis E. S., Ferl R. J., Sachs M. M., Peacock W. J. cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize. Proc Natl Acad Sci U S A. 1982 May;79(9):2981–2985. doi: 10.1073/pnas.79.9.2981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Glisin V., Crkvenjakov R., Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 1974 Jun 4;13(12):2633–2637. doi: 10.1021/bi00709a025. [DOI] [PubMed] [Google Scholar]
  11. Hake S., Kelley P. M., Taylor W. C., Freeling M. Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize. J Biol Chem. 1985 Apr 25;260(8):5050–5054. [PubMed] [Google Scholar]
  12. Harding J. D., MacDonald R. J., Przybyla A. E., Chirgwin J. M., Pictet R. L., Rutter W. J. Changes in the frequency of specific transcripts during development of the pancreas. J Biol Chem. 1977 Oct 25;252(20):7391–7397. [PubMed] [Google Scholar]
  13. Henle K. J., Dethlefsen L. A. Heat fractionation and thermotolerance: a review. Cancer Res. 1978 Jul;38(7):1843–1851. [PubMed] [Google Scholar]
  14. Lizardi P. M., Engelberg A. Rapid isolation of RNA using proteinase K and sodium perchlorate. Anal Biochem. 1979 Sep 15;98(1):116–122. doi: 10.1016/0003-2697(79)90714-0. [DOI] [PubMed] [Google Scholar]
  15. Luthe D. S., Quatrano R. S. Transcription in Isolated Wheat Nuclei: I. ISOLATION OF NUCLEI AND ELIMINATION OF ENDOGENOUS RIBONUCLEASE ACTIVITY. Plant Physiol. 1980 Feb;65(2):305–308. doi: 10.1104/pp.65.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Luthe D. S., Quatrano R. S. Transcription in Isolated Wheat Nuclei: II. CHARACTERIZATION OF RNA SYNTHESIZED IN VITRO. Plant Physiol. 1980 Feb;65(2):309–313. doi: 10.1104/pp.65.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pelham H. R., Bienz M. A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene. EMBO J. 1982;1(11):1473–1477. doi: 10.1002/j.1460-2075.1982.tb01340.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rowland L. J., Strommer J. N. Insertion of an unstable element in an intervening sequence of maize Adh1 affects transcription but not processing. Proc Natl Acad Sci U S A. 1985 May;82(9):2875–2879. doi: 10.1073/pnas.82.9.2875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sachs M. M., Freeling M., Okimoto R. The anaerobic proteins of maize. Cell. 1980 Jul;20(3):761–767. doi: 10.1016/0092-8674(80)90322-0. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

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