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. 2013 Jun 3;8(6):e63313. doi: 10.1371/journal.pone.0063313

Sequencing of Candidate Chromosome Instability Genes in Endometrial Cancers Reveals Somatic Mutations in ESCO1, CHTF18, and MRE11A

Jessica C Price 1, Lana M Pollock 1, Meghan L Rudd 1, Sarah K Fogoros 1, Hassan Mohamed 1, Christin L Hanigan 1, Matthieu Le Gallo 1, NIH Intramural Sequencing Center (NISC) Comparative Sequencing Program 2, Suiyuan Zhang 3, Pedro Cruz 3, Praveen F Cherukuri 3, Nancy F Hansen 3, Kirk J McManus 4, Andrew K Godwin 5,6, Dennis C Sgroi 7, James C Mullikin 2,3, Maria J Merino 8, Philip Hieter 9, Daphne W Bell 1,*
Editor: Todd W Miller10
PMCID: PMC3670891  PMID: 23755103

Abstract

Most endometrial cancers can be classified histologically as endometrioid, serous, or clear cell. Non-endometrioid endometrial cancers (NEECs; serous and clear cell) are the most clinically aggressive of the three major histotypes and are characterized by aneuploidy, a feature of chromosome instability. The genetic alterations that underlie chromosome instability in endometrial cancer are poorly understood. In the present study, we used Sanger sequencing to search for nucleotide variants in the coding exons and splice junctions of 21 candidate chromosome instability genes, including 19 genes implicated in sister chromatid cohesion, from 24 primary, microsatellite-stable NEECs. Somatic mutations were verified by sequencing matched normal DNAs. We subsequently resequenced mutated genes from 41 additional NEECs as well as 42 endometrioid ECs (EECs). We uncovered nonsynonymous somatic mutations in ESCO1, CHTF18, and MRE11A in, respectively, 3.7% (4 of 107), 1.9% (2 of 107), and 1.9% (2 of 107) of endometrial tumors. Overall, 7.7% (5 of 65) of NEECs and 2.4% (1 of 42) of EECs had somatically mutated one or more of the three genes. A subset of mutations are predicted to impact protein function. The co-occurrence of somatic mutations in ESCO1 and CHTF18 was statistically significant (P = 0.0011, two-tailed Fisher's exact test). This is the first report of somatic mutations within ESCO1 and CHTF18 in endometrial tumors and of MRE11A mutations in microsatellite-stable endometrial tumors. Our findings warrant future studies to determine whether these mutations are driver events that contribute to the pathogenesis of endometrial cancer.

Introduction

Uterine cancer is the most commonly diagnosed gynecologic malignancy in the United States and is the eighth leading cause of death from cancer among American women [1]. Endometrial cancers (ECs) account for the vast majority of uterine cancers. Endometrioid, serous, and clear cell carcinomas represent the three major histological subtypes of EC. Each subtype arises from distinct precursor lesions, has distinct clinical behaviors and distinct molecular etiologies [2], [3].

Endometrioid ECs (EECs) are estrogen-dependent tumors associated with an overall favorable prognosis evidenced by a 5-year relative survival rate of ∼90% [4]. In contrast, serous and clear cell ECs (non-endometrioid ECs (NEECs)) are clinically aggressive, estrogen-independent tumors with 5-year relative survival rates of only 44% and 65% respectively [4]. NEECs contribute disproportionately to mortality from EC. In a population-based study of endometrioid, serous, and clear cell ECs within the United States Surveillance Epidemiology and End Results (SEER) program (1988–2001), NEECs accounted for 47% of deaths even though they constituted only 13% of diagnoses [5].

EECs and NEECs exhibit distinct modes of genomic instability. EECs tend to be diploid or near-diploid but frequently exhibit microsatellite instability (MSI) [6], [7], [8], [9], [10], [11]. In contrast, NEECs are frequently aneuploid, or chromosomally unstable, but display MSI only rarely [11], [12], [13], [14], [15], [16], [17].

MSI reflects a mutator phenotype resulting from defective mismatch repair (reviewed in [18]). In sporadic endometrial cancers, most instances of MSI are explained by hypermethylation of the MLH1 promoter, loss of MSH2 expression, or somatic mutations in MSH6 (reviewed in [19]). Aneuploidy has recently been suggested to result from a step-wise process resulting from an acquired tolerance for a non-diploid genome, via inactivation of the p53 pathway, as well as aberrant chromosome segregation [20]. Although inactivating mutations in TP53 and p53 protein stabilization are frequent in NEECs, occurring in up to 90% of serous tumors (reviewed in [19]), the genetic basis of chromosome missegregation in NEECs remains poorly understood.

In yeast, chromosome missegregation can arise from mutations in genes that regulate sister-chromatid cohesion [21], [22]. Mitotic sister chromatid cohesion refers to the physical linkage of replicated sister chromatids by the cohesin protein complex until anaphase, to ensure the faithful segregation of sister chromatids into daughter cells. In S. cerevisiae, the cohesin complex consists of the Smc1, Smc3, Scc1, and Scc3 subunits and is loaded onto chromatin at the end of G1 by a process that requires the Scc2-Scc4 complex [23], [24], [25]. Subsequent cohesion establishment depends upon the acetylation of Smc3 by the Eco1 acetyltransferase [26], [27], [28], as well as the activities of Chl1 and the alternative replication factor C (Rfc) complex Ctf18-Ctf8-Dcc-Rfc [21], [29]. Cohesion establishment is antagonized by the activities of the Wpl1-Pds5 complex and the Elg1-Rfc complex [30], [31].

The proteins that regulate sister chromatid cohesion are highly conserved throughout evolution. In mammalian cells, the mitotic cohesin complex is formed by SMC1A (hSmc1), SMC3 (hSmc3), RAD21 (hScc1), and SA1/SA2 (hScc3). Cohesin loading is dependent upon NIPBL (hScc2) and MAU2 (hScc4) (reviewed in [32]). Cohesion establishment requires acetylation on SMC3 by the ESCO1 and ESCO2 acetyltransferases [33] and is also regulated by the CHTF18-RFC complex [34] and by DDX11 (hChl1) [35], [36].

There is a growing body of evidence implicating the mutational disruption of sister chromatid cohesion genes in human cancer. Somatic deletions and mutations of several genes that regulate sister chromatid cohesion have recently been uncovered in colorectal cancer, Ewing's sarcoma, glioblastoma, melanoma, acute myeloid leukemia, and myeloid diseases [37], [38], [39], [40], [41], [42], [43]. We previously described somatic loss-of-function mutations of ATAD5 in endometrial cancers [44]. ATAD5 is the human orthologue of S. cerevisiae Elg1, which forms an Rfc-like complex that participates in sister chromatid cohesion [45], [46].

In the present study, we sought to determine whether additional sister chromatid cohesion genes are somatically mutated in endometrial tumors. We resequenced the human orthologues of 19 genes implicated in the regulation of sister chromatid cohesion, as well as two additional candidate chromosome instability (CIN) genes, from 24 primary NEECs. Mutated genes were subsequently sequenced from 83 additional endometrial tumors. Our study uncovered nonsynonymous somatic mutations in ESCO1, CHTF18, and MRE11A in a subset of human endometrial tumors.

Materials and Methods

Ethics statement

The NIH Office of Human Subjects Research determined that this research was not “human subjects research” per the Common Rule (45 CFR 46), and therefore that no IRB review was required for sequencing of the anonymized samples in this study.

Clinical specimens

Anonymized, primary endometrial tumor tissues (45 serous, 20 clear cell, and 42 endometrioid) and matched histologically normal tissues were obtained from the Cooperative Human Tissue Network, or from the Biosample Repository at Fox Chase Cancer Center, Philadelphia PA. Six cases of matched tumor and normal DNAs were procured from Oncomatrix. All tumor tissues were collected before treatment. An hematoxylin and eosin (H&E) stained section of each tumor specimen was reviewed by a pathologist to verify histology and to delineate regions of tissue with high (≥70%) tumor cell content.

Nucleic acid isolation and identity testing

Genomic DNA was isolated from macrodissected tissue using the Puregene kit (Qiagen). Paired, tumor-normal DNAs were genotyped using the Coriell Identity Mapping kit (Coriell) according to the manufacturer's instructions. Genotyping fragments were size separated on an ABI-3730xl DNA analyzer (Applied Biosystems) and alleles were scored using GeneMapper (Applied Biosystems).

Identification of orthologous genes

A consolidated list of known and candidate human orthologues of yeast chromosome stability genes (with demonstrated roles in sister chromatid cohesion) was identified through standard cross-species approaches. Briefly, InParanoid 7 and HomoloGene databases were queried to identify known orthologues, while BLASTp was employed to identify the top-hit candidates (based on E-value) from the non-redundant protein sequences within the Homo sapiens database.

Reverse transcriptase PCR (RT-PCR)

Total RNA was extracted from 5 endometrioid and 2 serous endometrial cancer cell lines using Trizol Reagent (Ambion). A commercially available human total RNA control mix (Applied Biosystems) was used as a positive control. cDNA synthesis was performed on 1μg of total RNA with the high-capacity cDNA archive kit using random hexamers (Applied Biosystems). cDNAs (0.2μl) were amplified by PCR using the primer pairs provided in Table S1. Amplification consisted of 40 cycles using the following parameters: 94°C for 30s, 58°C for 30s and 72°C for 30s, with a final extension step at 72°C for 10min. PCR products were separated on a 1% agarose gel stained with ethidium bromide in 0.5× TAE buffer and visualized under ultraviolet illumination.

Cell lines and Western blot analysis

Serous endometrial cancer cell lines (ARK1 and ARK2) were kindly provided by Dr. Alessandro Santin (Yale School of Medicine). Endometrioid endometrial cancer cell lines (RL-95-2, HEC1A, HEC1B, ANC3A) and a cell line derived from a poorly differentiated endometrial adenocarcinoma (KLE) were obtained from the American Type Culture Collection, or the NCI Developmental Therapeutics Program cell line repository. Cells were washed in phosphate-buffered saline followed by lysis in ice-cold RIPA buffer (Thermo Scientific) containing 1mM Na-orthovanadate, 10mM NaF, and 1X protease inhibitor cocktail (Roche). Lysates were centrifuged and equal amounts of the cleared lysate were denatured at 95°C in 2× SDS sample buffer (Sigma) prior to SDS-PAGE and transfer to PVDF membranes (Bio-Rad). Primary and HRP-conjugated secondary antibodies were: αMRE-11 (Cell Signaling), αCHTF18 (Novus Biological), αESCO1 (Novus Biological), α-α/β-Tubulin (Cell Signaling), goat anti-mouse HRP (Cell Signaling), and goat anti-rabbit HRP (Cell Signaling). Immunoreactive proteins were visualized with enhanced chemiluminescence (Pierce).

Primer design and PCR amplification

Primer pairs were designed, using published methods [47], to target 97.4% (458 of 470) of all exons of the 21 genes in the mutation discovery screen (Table S2), and all exons of the three genes in the mutation prevalence screen (Table S3). PCR conditions are available on request.

Nucleotide sequencing

PCR products were subjected to bidirectional Sanger sequencing using M13 primers and the BigDye Terminator Version 3.1 Cycle Sequencing Kit (Applied Biosystems). Sequencing reactions were run on ABI 3730xl DNA Analyzers (Applied Biosystems). Sequence trace quality was assessed with the base-calling program, Phred [48], [49]. All traces were included in the subsequent analysis, since deletion-insertion polymorphisms can mimic poor quality data from a Phred-quality measure, but may contain valid sequence data. All sequences for a given primer pair were assembled using Consed [50]; overlapping amplimers were assembled separately to allow independent cross-validation of calls in overlapping regions. Sequence variants, including single-nucleotide differences and short (<100 base pair) insertions and deletions, were identified using PolyPhred v6.11 [51] and an in-house algorithm (DIPDetector) optimized for improved sensitivity in finding insertions and deletions from aligned trace data. DIPDetector analyzes Sanger sequencing traces and predicts insertions and deletions by first examining read alignments for homozygous variants. It then searches for signatures of heterozygous insertions and deletions within the output of the basecaller phred run with the – poly option [49]. After forming two vectors containing the bases with highest peak areas at each position of the read (or assigning the highest area peak to both vectors when the second largest peak has an area less than 10% the size of the largest peak), DIPDetector attempts to phase these vectors by inserting potential shifts of all possible sizes into all possible positions of the read, and scores these shifts according to how well the resulting shifted vectors match the observed bases within the trace. Human genome assembly hg18 (NCBI Build 36.1) was used as the reference sequence. Variant positions were cross-referenced to dbSNP (Build 129) entries to identify known polymorphisms. To determine whether novel variants were somatic mutations or germline polymorphisms, the appropriate tumor DNA and matched normal DNA were re-amplified in an independent PCR followed by sequence analysis of the variant position. The predicted impact of somatic mutations on protein function was evaluated in silico using Mutation Assessor release 2 (http://mutationassessor.org/), SIFT (http://sift.jcvi.org/), and Polyphen-2 (http://genetics.bwh.harvard.edu/pph2/index.shtml).

Calculation of discovery screen power

The estimated power to detect one gene mutation in a set of 24 tumors is 1– (1-X)?24, where X is the actual fraction of tumors with a mutation in that gene.

Results and Discussion

In a mutation discovery screen, we analyzed 24 primary NEECs for the presence of nucleotide variants within the coding exons and splice junctions of 21 candidate chromosome instability genes, which are expressed, at variable levels, in endometrial cancer cell lines (Figure S1). Nineteen of these genes are implicated in the regulation of sister-chromatid cohesion, based on their sequence homology to cohesion genes in S. cerevisiae ( Table 1 ). The 24 NEECs consisted of 17 serous ECs and 7 clear cell ECs; five of the serous tumors (T33, T45, T65, T69, T70) were recently subjected to whole exome sequencing [52]. We included only MSI-stable tumors in the discovery screen; the MSI data have been reported elsewhere [52].

Table 1. Genes resequenced in the mutation discovery screen.

Human Gene Symbol Human Gene Name Human mRNA Accession Number Human Protein Accession Number S. cerevisiae Ortholog E- value S. cerevisiae Ortholog Regulates Cohesion
APRIN/PDS5B PDS5, regulator of cohesion maintenance, homolog B NM_015032.1 NP_055847.1 PDS5 2E-32 Yes
CHTF8/DERPC CTF8, chromosome transmission fidelity factor 8 homolog NM_001039690 NP_001035236.1 CTF8 NA Yes
CHTF18 CTF18, chromosome transmission fidelity factor 18 homolog NM_022092.1 NP_071375.1 CTF18 8E-42 Yes
CSPG6/SMC3 Structural maintenance of chromosomes 3 NM_005445.3 NP_005436.1 SMC3 0.0 Yes
DDX11/CHLR1 DEAD/H (Asp-Glu-Ala- Asp/His) box polypeptide 11 NM_030653.2 NP_085911.2 CHL1 2E-139 Yes
DSCC1/DCC1 Defective in sister chromatid cohesion 1 homolog NM_024094.1 NP_076999.2 DCC1 8E-11 Yes
ESCO1 Establishment of cohesion 1 homolog 1 NM_052911.1 NP_443143.2 ECO1/CTF7 8E-15 Yes
KIAA1212/CCDC88A Coiled-coil domain containing 88A NM_018084.3 NP_060554.3 RAD61 4E-04 Yes
LEO1 Leo1, Paf1/RNA polymerase II complex component, homolog NM_138792.2 NP_620147.1 MRC1 1E-04 -
MRE11A MRE11 meiotic recombination 11 homolog A NM_005591 NP_005582.1 MRE11 2E-130 Yes
NIPBL Nipped-B homolog NM_015384.3 NP_597677.2 SCC2 4E-14 Yes
REC8L1 REC8 homolog NM_001048205.1 NP_005123.1 SPO69 9E-05 Yes
SCC-112/PDS5A PDS5, regulator of cohesion maintenance, homolog A NM_015200 NP_056015 PDS5 6E-32 Yes
SGOL1 Shugoshin-like 1 NM_001012409.1 NP 001012410.1 SGO1 NA Yes
SMC1L1/SMC1A Structural maintenance of chromosomes 1A NM_006306.2 NP_006297.2 SMC1 1E-153 Yes
STAG2 Stromal antigen 2 NM_006603 NP_006594.3 SCC3 5E-22 Yes
STAG3 2 Stromal antigen 3 NM_012447.2 NP_036579.2 SCC3 8E-23 Yes
TIMELESS Timeless homolog (Drosophila) NM_003920.1 NP_003911.2 TOF1 1E-11 Yes
TIPIN TIMELESS interacting protein NM_017858.1 NP_060328.2 CSM3 7E-10 Yes
WDHD1/hCTF4 WD repeat and HMG-box DNA binding protein 1 NM_007086.1 NP_009017.1 CTF4 7E-21 Yes
ZC3H13/DING 1 Zinc finger CCCH-type containing 13 NM_015070 NP_055885.2 PDS1 - -
1

Gene analyzed because it is somatically mutated in colorectal cancer.

2

Implicated in meiotic specific cohesion.

We obtained high quality sequence data for 87.6% (5.64Mb) of bases (6.44Mb) targeted. After excluding variants that were annotated as single nucleotide polymorphisms (SNPs) within dbSNP (Build 129), there were 109 unique nucleotide variants that represented potential somatic mutations. To determine whether these variants were somatic mutations or germline variants, we reamplified and sequenced the variant positions from the appropriate tumor DNA and matched normal DNA. Three variants were bone fide somatic mutations, present in the tumor DNA but absent from the matched normal DNA. The somatically mutated genes were ESCO1 (establishment of cohesion 1 homolog 1 (S. cerevisiae)), CHTF18 (chromosome transmission fidelity factor 18 homolog (S. cerevisiae)), and MRE11A (meiotic recombination 11 homolog A (S. cerevisiae)); each gene was mutated in 4% (1 of 24) of NEECs in the discovery screen. Although we found no evidence for somatic mutations in the remaining 18 candidate CIN genes, it is important to acknowledge that our discovery screen has insufficient power to detect all somatic mutations present in NEECs. We estimate that in a screen of 24 NEECs, the power to detect genes that are somatically mutated in 5%, 10% or 15% of all NEECs is 71%, 92%, and 98% respectively.

We next sought to more precisely determine the frequency and spectrum of somatic mutations in ESCO1, CHTF18, and MRE11A in endometrial cancer. To do this, we performed a prevalence screen in which we resequenced the coding exons and splice sites of the three genes from an additional 28 serous tumors, 13 clear cell tumors, and 42 endometrioid tumors, unselected for MSI status.

In the combined discovery and prevalence screens, we uncovered nonsynonymous somatic mutations within ESCO1, CHTF18, and MRE11A in, respectively, 3.7% (4 of 107), 1.9% (2 of 107), and 1.9% (2 of 107) of endometrial tumors ( Table 2 and Figure S2). Overall, 7.7% (5 of 65) of NEECs and 2.4% (1 of 42) of EECs had somatic mutations in one or more of the three genes. Compared to known consensus cancer genes with established roles in endometrial cancer, and to significantly mutated cancer genes, ESCO1, CHTF18, and MRE11A were infrequently mutated (Figure S3, Figure S4, Figure S5) [44], [52], [53], [54], suggesting that these three genes are either rare pathogenic driver genes for endometrial cancer or that they are non-pathogenic genes that have acquired passenger mutations. Immunoblotting confirmed the expression of MRE11A and CHTF18 in panel of endometrial cancer cell lines (Figure S6); ESCO1 was variably expressed among these same cell lines.

Table 2. Nonsynonymous, somatic mutations in ESCO1, CHTF18 and MRE11A, in ECs.

Gene Case No. Histological Subtype MSI Status Nucleotide Change § Amino Acid Change Mutation Type Mutation Assessor (Release 2) Prediction SIFT Prediction Polyphen-2 Prediction
ESCO1 T79 Serous Stable c.G1012T p.E338X Nonsense n/a n/a n/a
T113 Clear cell Stable c.G1075A p.D359N Missense Low Tolerated Benign
T77 Clear cell Unstable c.C1595T p.S532L Missense Low Tolerated Probably damaging
T88 Endometrioid Unstable c.C2356T p.R786C Missense Medium Affects function Probably damaging
CHTF18 T79 Serous Stable c.G2035A p.D679N Missense Low Tolerated Probably damaging
T88 Endometrioid Unstable c.C2560T p.R854W Missense Medium Tolerated Possibly damaging
MRE11A T68 Serous Stable c.G2074T p.D692Y Missense Low Affects function Probably damaging
T3 Serous Stable c.G1448A p.R483Q Missense Low Tolerated Benign
T3 Serous Stable c.G391A p.D131N Missense Medium Affects function Possibly damaging

Case no T3 is also known as OM-1323.

n/a Not applicable.

§

Transcript accession numbers: ESCO1 (NM_052911.1), CHTF18 (NM_022092.1), MRE11A (NM_005591).

Protein accession numbers: ESCO1 (NP_443143.2), CHTF18 (NP_071375.1), MRE11A (NP_005582.1).

ESCO1, which encodes a lysine acetyltransferase that is essential for the establishment of sister chromatid cohesion in mammalian cells, was somatically mutated in 2.2% (1 of 45) of serous ECs, 10% (2 of 20) of clear cell ECs, and 2.4% (1 of 42) of endometrioid ECs. Two of the ESCO1 mutations are predicted to impact protein function. The ESCO1R786C missense mutant, within the acetyltransferase domain, is predicted to impact protein function by both the SIFT and Polyphen algorithms ( Table 2 ). We speculate that the ESCO1E338X nonsense mutant, which we uncovered in a serous-EC, may be a loss-of function mutant since a protein produced by this allele would be prematurely truncated and fail to include the acetyltransferase domain. Alternatively, nonsense-mediated decay of the ESCO1E338X transcript might lead to haploinsufficiency.

CHTF18 was somatically mutated in 2.2% (1 of 45) of serous ECs and 2.4% (1 of 42) of endometrioid ECs. In human cells, the CHTF18-RFC complex regulates the acetylation of the SMC3 cohesion-subunit by ESCO1 and ESCO2 acetyltransferases [34], thereby contributing to the establishment of sister chromatid cohesion. The CHTF18-RFC complex has also been implicated in the stimulation of DNA polymerase η activity, and in the recruitment of DNA polymerase ε to sites of gap-filling repair synthesis [55], [56]. Both of the CHTF18 mutants we uncovered in endometrial cancer localize to the carboxy-terminus of the protein ( Figure 1 ), within a region (residues 576-876) that mediates binding to RFC2-5 [57]. The CHTF18R854W mutant is predicted to possibly affect protein function by the Mutation Assessor and SIFT algorithms ( Table 2 ). Interestingly, the majority of CHTF18 mutations observed in other cancers also localize to the C-terminus of the encoded protein [58]. These observations raise the possibility that somatic missense mutations in the C-terminus of CHTF18, found here and in other cancers, might disrupt the CHTF18-RFC interaction.

Figure 1. Localization of somatic mutations in ESCO1, CHTF18, and MRE11A in primary endometrial tumors, relative to important functional domains of the encoded proteins.

Figure 1

Individual somatic mutations are indicated by squares (nonsense mutations) or diamonds (missense mutations). Domain positions are derived from [65], [66], [61], [59], [67]. GAR: Glycine-Arginine-Rich motif; RBD:RAD50 Binding Domain; RFC box: Replication Factor C box.

MRE11A was somatically mutated in 4.4% (2 of 45) of serous ECs. No MRE11A mutations were observed among clear cell or endometrioid tumors. MRE11A possesses both endonuclease activity and 3′–5′ exonuclease activity and, as a component the MRE11A-RAD50-NBS1 (MRN) complex, it plays an essential role in the cellular response to double strand breaks (reviewed in [59]). In mammalian cells, the MRN complex is also required for ATR-mediated phosphorylation of the SMC1 subunit of cohesin [60], and siRNA depletion of MRE11A in human cells results in cohesion defects [37]. The MRE11AD131N somatic mutant, which we uncovered in a serous EC, occurs at a highly evolutionarily conserved residue in the third phosphoesterase motif within the nuclease domain [61] and is predicted to impact protein function ( Figure 1 , and Table 2 ). The MRE11AD692Y mutant, in the DNA binding domain, is also predicted to be functionally significant ( Table 2 ). Although intronic somatic mutations in MRE11A have been reported in microsatellite unstable endometrial cancers [62], [63], [64], to our knowledge, the present study is the first report of somatic mutations of MRE11A in microsatellite stable endometrial tumors ( Table 2 ). Of note, the MRE11AD131N variant, which was somatic in our study, has also been observed as a rare population variant (TMP_ESP_11_94212851) in the NHLBI Exome Sequencing Project (URL: http://evs.gs.washington.edu/EVS/), with a minor allele frequency of 0.0233% in the EuropeanAmerican population.

The mutual exclusivity or co-occurrence of somatic mutations in two or more genes can indicate functional redundancy or functional synergy, respectively. To determine the pattern of somatic mutations within cohesion genes in endometrial cancer, we combined the results of the present study with our previous analysis of the ATAD5 (hELG1) gene in this same cohort of ECs [44]. Although the number of mutated cases is small, we observed that somatic mutations in ESCO1 and ATAD5 tended to co-occur in endometrial cancer (P = 0.0102, two-tailed Fisher's exact test), as did somatic mutations in ESCO1 and CHTF18 (P = 0.0011) ( Figure 2 , and Table 3 ). These observations raise the possibility that there might be functional synergy between ESCO1 and ATAD5 mutants, and between ESCO1 and CHTF18 mutants, in endometrial cancer. In this regard, it is noteworthy that somatic mutations in ESCO1 and ATAD5 tend to also co-occur in colorectal tumors (P = 0.000001) (Figure S7), based on an analysis of the publically available mutation data generated by The Cancer Genome Atlas [http://cbio.mskcc.org/cancergenomics/]. An alternative, but not mutually exclusive, possibility is that the co-occurring mutations of cohesion genes in endometrial cancer may reflect an underlying hypermutable phenotype. We previously evaluated the cohort of 107 tumors in this study for microsatellite instability and MSH6 mutations [44], [52], both of which can give rise to hypermutability due to defective mismatch repair (MMR). Although three of the tumors with cohesion gene mutations in this study were either MSI-unstable or MSH6-mutated ( Figure 2 ), we observed no statistically significant association between mutations in sister chromatid cohesion genes and defects in mismatch repair (Table S4 and Table S5).

Figure 2. Oncoprint displaying nonsynonymous somatic mutations in ESCO1, CHTF18, MRE11A, and ATAD5 in eight primary endometrial cancers.

Figure 2

Individual tumors (T) are indicated by vertical gray bars. Tumors consist of NEECs (T3, T51, T62, T68, T77, T79, T113) and an EEC (T88). Genes (left) and nonsynonymous somatic mutations (orange boxes) are indicated. ESCO1, CHTF18, and MRE11A were analyzed in this study; *ATAD5 mutations, MSH6 mutations, and microsatellite instability (MSI) have previously been described elsewhere [44], [52].

Table 3. Co-occurrence of ESCO1 mutations with CHTF18 or ATAD5 mutations in EC.

Mutation Status No. of ESCO1-mutated Cases (%) P-value§
CHTF18-mutated (n = 2) 2 (100%) P = 0.0011
CHTF18-nonmutated (n = 105) 2 (1.90%)
ATAD5-mutated (n = 5) 2 (40%) P = 0.0102
ATAD5-nonmutated (n = 102) 2 (1.96%)
§

Two-tailed Fisher's exact test.

In summary, we have identified rare, nonsynonymous, somatic mutations within ESCO1, CHTF18, and MRE11A in a subset of primary endometrial tumors. Future studies will be required to determine whether these mutations are driver events that contribute to the pathogenesis of endometrial cancer.

Supporting Information

Figure S1

RT-PCR analysis of 21 candidate human chromosomal instability genes in 7 human endometrial cancer cell lines. Gel electrophoresis of RT-PCR products confirms the expression of the 21 candidate chromosome instability genes in serous and endometrioid endometrial cancer cell lines. Positive and negative (water) PCR controls are shown. ACTB and GAPDH served as positive control genes.

(TIF)

Figure S2

Sequence chromatograms showing somatic mutations in ESCO1 , CHTF18 , and MRE11A in endometrial tumor DNAs, compared to the matched normal DNAs.

(TIF)

Figure S3

Oncoprints displaying the distribution of somatic mutations in serous endometrial tumors as reported in this study (*) and elsewhere [44] , [52] , [53] , [54] . Each blue bar represents an individual tumor (T). Nonsynonymous somatic mutations and MSI+ are indicated by the red bars. For MSH6, germline variants of unknown functional significance are displayed by orange bars. The observed frequency (%) of mutated cases, for each gene, is shown on the right.

(TIF)

Figure S4

Oncoprints displaying the distribution of somatic mutations in clear cell endometrial tumors as reported in this study (*) and elsewhere [44] , [52] , [53] , [54] . Each blue bar represents an individual tumor (T). Nonsynonymous somatic mutations and MSI+ are indicated by the red bars. For MSH6, a germline variant of unknown functional significance is displayed by the orange bar. The observed frequency (%) of mutated cases, for each gene, is shown on the right.

(TIF)

Figure S5

Oncoprints displaying the distribution of somatic mutations in endometrioid endometrial tumors as reported in this study (*) and elsewhere [44] , [52] , [53] , [54] . Each blue bar represents an individual tumor (T). Nonsynonymous somatic mutations and MSI+ are indicated by the red bars. For MSH6, germline variants of unknown functional significance are displayed by orange bars. The observed frequency (%) of mutated cases, for each gene, is shown on the right.

(TIF)

Figure S6

Immunoblots showing expression levels of the MRE11A, CHTF18 and ESCO1 proteins among a panel of 7 human endometrial cancer cell lines. Tubulin was used as a control for protein loading.

(TIF)

Figure S7

Oncoprint displaying patterns of somatic mutations in ESCO1 , CHTF18 , MRE11A , and ATAD5 in colorectal cancer, as reported by The Cancer Genome Atlas (TCGA). (Upper panel) Individual colorectal tumors are indicated by vertical gray bars. Genes (left) and nonsynonymous somatic mutations (orange bars) are indicated. (Lower panel) In colorectal cancers, mutations in ATAD5 and ESCO1 showed a strong tendency towards co-occurrence; mutations in MRE11A and ESCO1, and in ATAD5 and MRE11A showed a tendency towards co-occurrence. The data were derived from 224 sequenced samples; the TCGA data were accessed, and the mutual exclusivity calculated via the cBio Cancer Genomics Portal (http://www.cbioportal.org/public-portal/).

(TIF)

Table S1

RT-PCR primers used to assess the expression of 21 candidate human chromosomal instability genes.

(XLSX)

Table S2

PCR primers used to amplify 21 candidate human chromosomal instability genes within the discovery screen.

(DOC)

Table S3

PCR primers used to amplify and sequence CHTF18, ESCO1, and MRE11A within the validation screen.

(DOC)

Table S4

Status of microsatellite instability, MSH6 , ESCO1 , CHTF18 , MRE11A , and ATAD5 for the 107 endometrial tumors in this study.

(XLSX)

Table S5

Frequency of somatic mutations in the ESCO1 , CHTF18 , MRE11A , and ATAD5 cohesion genes in 105 endometrial tumors, according to microsatellite instability and MSH6 status.

(XLSX)

Acknowledgments

We thank our colleagues for careful reading of the manuscript and thoughtful discussion.

Funding Statement

This work was funded, in part, by the Intramural Research Program of the National Human Genome Research Institute at NIH (DWB, JCM); NIH grant CA016519 (PH); Canadian Institutes of Health Research (CIHR) grant MOP-38096 (PH); Manitoba Health Research Council (MHRC) grant (KJM); CIHR/MHRC RPP New Investigator award (KJM); U01 CA113916 and R01 CA140323 (AKG); NIH RO1-1CA112021-01 (DCS); the NCI SPORE in breast cancer at Massachusetts General Hospital (DCS); and the Avon Foundation (DCS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Figure S1

RT-PCR analysis of 21 candidate human chromosomal instability genes in 7 human endometrial cancer cell lines. Gel electrophoresis of RT-PCR products confirms the expression of the 21 candidate chromosome instability genes in serous and endometrioid endometrial cancer cell lines. Positive and negative (water) PCR controls are shown. ACTB and GAPDH served as positive control genes.

(TIF)

Figure S2

Sequence chromatograms showing somatic mutations in ESCO1 , CHTF18 , and MRE11A in endometrial tumor DNAs, compared to the matched normal DNAs.

(TIF)

Figure S3

Oncoprints displaying the distribution of somatic mutations in serous endometrial tumors as reported in this study (*) and elsewhere [44] , [52] , [53] , [54] . Each blue bar represents an individual tumor (T). Nonsynonymous somatic mutations and MSI+ are indicated by the red bars. For MSH6, germline variants of unknown functional significance are displayed by orange bars. The observed frequency (%) of mutated cases, for each gene, is shown on the right.

(TIF)

Figure S4

Oncoprints displaying the distribution of somatic mutations in clear cell endometrial tumors as reported in this study (*) and elsewhere [44] , [52] , [53] , [54] . Each blue bar represents an individual tumor (T). Nonsynonymous somatic mutations and MSI+ are indicated by the red bars. For MSH6, a germline variant of unknown functional significance is displayed by the orange bar. The observed frequency (%) of mutated cases, for each gene, is shown on the right.

(TIF)

Figure S5

Oncoprints displaying the distribution of somatic mutations in endometrioid endometrial tumors as reported in this study (*) and elsewhere [44] , [52] , [53] , [54] . Each blue bar represents an individual tumor (T). Nonsynonymous somatic mutations and MSI+ are indicated by the red bars. For MSH6, germline variants of unknown functional significance are displayed by orange bars. The observed frequency (%) of mutated cases, for each gene, is shown on the right.

(TIF)

Figure S6

Immunoblots showing expression levels of the MRE11A, CHTF18 and ESCO1 proteins among a panel of 7 human endometrial cancer cell lines. Tubulin was used as a control for protein loading.

(TIF)

Figure S7

Oncoprint displaying patterns of somatic mutations in ESCO1 , CHTF18 , MRE11A , and ATAD5 in colorectal cancer, as reported by The Cancer Genome Atlas (TCGA). (Upper panel) Individual colorectal tumors are indicated by vertical gray bars. Genes (left) and nonsynonymous somatic mutations (orange bars) are indicated. (Lower panel) In colorectal cancers, mutations in ATAD5 and ESCO1 showed a strong tendency towards co-occurrence; mutations in MRE11A and ESCO1, and in ATAD5 and MRE11A showed a tendency towards co-occurrence. The data were derived from 224 sequenced samples; the TCGA data were accessed, and the mutual exclusivity calculated via the cBio Cancer Genomics Portal (http://www.cbioportal.org/public-portal/).

(TIF)

Table S1

RT-PCR primers used to assess the expression of 21 candidate human chromosomal instability genes.

(XLSX)

Table S2

PCR primers used to amplify 21 candidate human chromosomal instability genes within the discovery screen.

(DOC)

Table S3

PCR primers used to amplify and sequence CHTF18, ESCO1, and MRE11A within the validation screen.

(DOC)

Table S4

Status of microsatellite instability, MSH6 , ESCO1 , CHTF18 , MRE11A , and ATAD5 for the 107 endometrial tumors in this study.

(XLSX)

Table S5

Frequency of somatic mutations in the ESCO1 , CHTF18 , MRE11A , and ATAD5 cohesion genes in 105 endometrial tumors, according to microsatellite instability and MSH6 status.

(XLSX)


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