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. 1986 Nov;6(11):3965–3972. doi: 10.1128/mcb.6.11.3965

Circumsporozoite protein of Plasmodium berghei: gene cloning and identification of the immunodominant epitopes.

D J Eichinger, D E Arnot, J P Tam, V Nussenzweig, V Enea
PMCID: PMC367160  PMID: 2432395

Abstract

The gene encoding the circumsporozoite (CS) protein of the rodent malaria parasite Plasmodium berghei was cloned and characterized. A cDNA library made from P. berghei sporozoite RNA was screened with a monoclonal antibody for expression of CS protein epitopes. The resulting cDNA clone was used to isolate the CS protein gene from a lambda library containing parasite blood-stage DNA. The CS protein gene contains a central region encoding two types of tandemly repeated amino acid units, flanked by nonrepeated regions encoding amino- and carboxy-terminal signal and anchorlike sequences, respectively. One of the central repeated amino acid unit types contains the immunodominant epitopes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aikawa M., Yoshida N., Nussenzweig R. S., Nussenzweig V. The protective antigen of malarial sporozoites (Plasmodium berghei) is a differentiation antigen. J Immunol. 1981 Jun;126(6):2494–2495. [PubMed] [Google Scholar]
  2. Arnot D. E., Barnwell J. W., Tam J. P., Nussenzweig V., Nussenzweig R. S., Enea V. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science. 1985 Nov 15;230(4727):815–818. doi: 10.1126/science.2414847. [DOI] [PubMed] [Google Scholar]
  3. Dame J. B., Williams J. L., McCutchan T. F., Weber J. L., Wirtz R. A., Hockmeyer W. T., Maloy W. L., Haynes J. D., Schneider I., Roberts D. Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum. Science. 1984 Aug 10;225(4662):593–599. doi: 10.1126/science.6204383. [DOI] [PubMed] [Google Scholar]
  4. Ellis J., Ozaki L. S., Gwadz R. W., Cochrane A. H., Nussenzweig V., Nussenzweig R. S., Godson G. N. Cloning and expression in E. coli of the malarial sporozoite surface antigen gene from Plasmodium knowlesi. Nature. 1983 Apr 7;302(5908):536–538. doi: 10.1038/302536a0. [DOI] [PubMed] [Google Scholar]
  5. Enea V., Arnot D., Schmidt E. C., Cochrane A., Gwadz R., Nussenzweig R. S. Circumsporozoite gene of plasmodium cynomolgi (Gombak):cDNA cloning and expression of the repetitive circumsporozoite epitope. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7520–7524. doi: 10.1073/pnas.81.23.7520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Enea V., Ellis J., Zavala F., Arnot D. E., Asavanich A., Masuda A., Quakyi I., Nussenzweig R. S. DNA cloning of Plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope. Science. 1984 Aug 10;225(4662):628–630. doi: 10.1126/science.6204384. [DOI] [PubMed] [Google Scholar]
  7. Enea V., Galinski M., Schmidt E., Gwadz R., Nussenzweig R. S. Evolutionary profile of the circumsporozoite gene of the Plasmodium cynomolgi complex. J Mol Biol. 1986 Apr 20;188(4):721–726. doi: 10.1016/s0022-2836(86)80017-1. [DOI] [PubMed] [Google Scholar]
  8. Enea V. The circumsporozoite antigen: the gene and the antigen. Ann Ist Super Sanita. 1985;21(3):291–298. [PubMed] [Google Scholar]
  9. Ferreira A., Enea V., Morimoto T., Nussenzweig V. Infectivity of Plasmodium berghei sporozoites measured with a DNA probe. Mol Biochem Parasitol. 1986 May;19(2):103–109. doi: 10.1016/0166-6851(86)90114-3. [DOI] [PubMed] [Google Scholar]
  10. Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
  11. Gysin J., Barnwell J., Schlesinger D. H., Nussenzweig V., Nussenzweig R. S. Neutralization of the infectivity of sporozoites of Plasmodium knowlesi by antibodies to a synthetic peptide. J Exp Med. 1984 Sep 1;160(3):935–940. doi: 10.1084/jem.160.3.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  13. Hockmeyer W. T., Dame J. B. Recent efforts in the development of a sporozoite vaccine against human malaria. Adv Exp Med Biol. 1985;185:233–245. doi: 10.1007/978-1-4684-7974-4_16. [DOI] [PubMed] [Google Scholar]
  14. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  15. Loenen W. A., Brammar W. J. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene. 1980 Aug;10(3):249–259. doi: 10.1016/0378-1119(80)90054-2. [DOI] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mazier D., Mellouk S., Beaudoin R. L., Texier B., Druilhe P., Hockmeyer W., Trosper J., Paul C., Charoenvit Y., Young J. Effect of antibodies to recombinant and synthetic peptides on P. falciparum sporozoites in vitro. Science. 1986 Jan 10;231(4734):156–159. doi: 10.1126/science.3510455. [DOI] [PubMed] [Google Scholar]
  18. Nelson T., Brutlag D. Addition of homopolymers to the 3'-ends of duplex DNA with terminal transferase. Methods Enzymol. 1979;68:41–50. doi: 10.1016/0076-6879(79)68005-9. [DOI] [PubMed] [Google Scholar]
  19. Nussenzweig R. S., Vanderberg J., Most H., Orton C. Protective immunity produced by the injection of x-irradiated sporozoites of plasmodium berghei. Nature. 1967 Oct 14;216(5111):160–162. doi: 10.1038/216160a0. [DOI] [PubMed] [Google Scholar]
  20. Nussenzweig R., Vanderberg J., Most H. Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. IV. Dose response, specificity and humoral immunity. Mil Med. 1969 Sep;134(10):1176–1182. [PubMed] [Google Scholar]
  21. Ozaki L. S., Svec P., Nussenzweig R. S., Nussenzweig V., Godson G. N. Structure of the plasmodium knowlesi gene coding for the circumsporozoite protein. Cell. 1983 Oct;34(3):815–822. doi: 10.1016/0092-8674(83)90538-x. [DOI] [PubMed] [Google Scholar]
  22. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Santoro F., Cochrane A. H., Nussenzweig V., Nardin E. H., Nussenzweig R. S., Gwadz R. W., Ferreira A. Structural similarities among the protective antigens of sporozoites from different species of malaria parasites. J Biol Chem. 1983 Mar 10;258(5):3341–3345. [PubMed] [Google Scholar]
  25. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ullrich A., Shine J., Chirgwin J., Pictet R., Tischer E., Rutter W. J., Goodman H. M. Rat insulin genes: construction of plasmids containing the coding sequences. Science. 1977 Jun 17;196(4296):1313–1319. doi: 10.1126/science.325648. [DOI] [PubMed] [Google Scholar]
  28. Vanderberg J. P., Nussenzweig R. S., Most H. Further studies on the Plasmodium berghei-Anopheles stephensi--rodent system of mammalian malaria. J Parasitol. 1968 Oct;54(5):1009–1016. [PubMed] [Google Scholar]
  29. Vanderberg J., Nussenzweig R., Most H. Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. V. In vitro effects of immune serum on sporozoites. Mil Med. 1969 Sep;134(10):1183–1190. [PubMed] [Google Scholar]
  30. Vergara U., Ruiz A., Ferreira A., Nussenzweig R. S., Nussenzweig V. Conserved group-specific epitopes of the circumsporozoite proteins revealed by antibodies to synthetic peptides. J Immunol. 1985 May;134(5):3445–3448. [PubMed] [Google Scholar]
  31. Yoshida N., Potocnjak P., Nussenzweig V., Nussenzweig R. S. Biosynthesis of Pb44, the protective antigen of sporozoites of Plasmodium berghei. J Exp Med. 1981 Oct 1;154(4):1225–1236. doi: 10.1084/jem.154.4.1225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zavala F., Cochrane A. H., Nardin E. H., Nussenzweig R. S., Nussenzweig V. Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes. J Exp Med. 1983 Jun 1;157(6):1947–1957. doi: 10.1084/jem.157.6.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Zavala F., Tam J. P., Hollingdale M. R., Cochrane A. H., Quakyi I., Nussenzweig R. S., Nussenzweig V. Rationale for development of a synthetic vaccine against Plasmodium falciparum malaria. Science. 1985 Jun 21;228(4706):1436–1440. doi: 10.1126/science.2409595. [DOI] [PubMed] [Google Scholar]

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