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. 1986 Nov;6(11):4093–4098. doi: 10.1128/mcb.6.11.4093

Sequences involved in accurate and efficient transcription of human c-myc genes microinjected into frog oocytes.

K Nishikura
PMCID: PMC367176  PMID: 3025632

Abstract

By microinjecting a series of deletion mutant constructs into Xenopus laevis oocytes, transcriptional control regions, two promoters, of the human c-myc gene were defined. In the case of the first promoter, sequences between -60 and -37 relative to the transcription start site contained an element essential for promoter activity. In the case of the second promoter, sequences between -66 and -56 relative to the initiation site appeared to be involved in accurate and efficient transcription. In both cases, the region identified as the essential promoter element contained GGGCGG or GGCGGG,GC box-like sequences, suggesting that c-myc gene promoter activity may be controlled by transcription factor Sp1 binding in the microinjected oocytes.

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Selected References

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