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. 1986 Nov;6(11):4122–4125. doi: 10.1128/mcb.6.11.4122

Active X chromosome DNA is unmethylated at eight CCGG sites clustered in a guanine-plus-cytosine-rich island at the 5' end of the gene for phosphoglycerate kinase.

D H Keith, J Singer-Sam, A D Riggs
PMCID: PMC367182  PMID: 3025634

Abstract

The 5' control region and first exon for human X-chromosome-linked phosphoglycerate kinase is contained in a G + C-rich island. We measured methylation at all HpaII sites in this 5' region of leukocyte DNA. By use of a blotting procedure that allows analysis of small DNA fragments, we found that the HpaII sites are entirely methylated when from an inactive X chromosome and entirely unmethylated when from an active one. In contrast, methylation of HpaII sites in more downstream regions of the gene is essentially the same in active and inactive X chromosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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