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. 1986 Dec;6(12):4329–4334. doi: 10.1128/mcb.6.12.4329

Substrate recognition by the 2 micron circle site-specific recombinase: effect of mutations within the symmetry elements of the minimal substrate.

P V Prasad, D Horensky, L J Young, M Jayaram
PMCID: PMC367214  PMID: 3796604

Abstract

The minimal substrate for the 2 microns circle site-specific recombinase FLP consists of a nearly perfect 13-base-pair dyad symmetry with an 8-base-pair core. By using a series of chemically synthesized FLP substrates in in vitro FLP recombination and FLP-binding assays, we have identified four positions within each of the symmetry elements that are important contact points for the FLP protein. Furthermore, the binding and recombination data provide evidence for cooperativity between the two symmetry elements of a substrate and between the symmetry elements of two partner substrates during FLP recombination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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