Abstract
A gene isolated by screening Drosophila melanogaster tissue culture cells for DNA damage regulation was also found to be regulated by heat shock. After UV irradiation or heat shock, induction is at the transcriptional level and results in the accumulation of a 1.0-kilobase polyadenylated transcript. The restriction map of the clone bears no resemblance to the known heat shock genes, which are shown to be uninduced by UV irradiation.
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Angel P., Pöting A., Mallick U., Rahmsdorf H. J., Schorpp M., Herrlich P. Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts. Mol Cell Biol. 1986 May;6(5):1760–1766. doi: 10.1128/mcb.6.5.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craig E. A. The heat shock response. CRC Crit Rev Biochem. 1985;18(3):239–280. doi: 10.3109/10409238509085135. [DOI] [PubMed] [Google Scholar]
- Fyrberg E. A., Kindle K. L., Davidson N., Kindle K. L. The actin genes of Drosophila: a dispersed multigene family. Cell. 1980 Feb;19(2):365–378. doi: 10.1016/0092-8674(80)90511-5. [DOI] [PubMed] [Google Scholar]
- Fyrberg E. A., Mahaffey J. W., Bond B. J., Davidson N. Transcripts of the six Drosophila actin genes accumulate in a stage- and tissue-specific manner. Cell. 1983 May;33(1):115–123. doi: 10.1016/0092-8674(83)90340-9. [DOI] [PubMed] [Google Scholar]
- Jorritsma J. B., Konings A. W. The occurrence of DNA strand breaks after hyperthermic treatments of mammalian cells with and without radiation. Radiat Res. 1984 Apr;98(1):198–208. [PubMed] [Google Scholar]
- Krueger J. H., Walker G. C. groEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR+-dependent fashion. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1499–1503. doi: 10.1073/pnas.81.5.1499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lis J. T., Neckameyer W., Dubensky R., Costlow N. Cloning and characterization of nine heat-shock-induced mRNAs of Drosophila melanogaster. Gene. 1981 Oct;15(1):67–80. doi: 10.1016/0378-1119(81)90105-0. [DOI] [PubMed] [Google Scholar]
- Love J. D., Minton K. W. Screening of lambda library for differentially expressed genes using in vitro transcripts. Anal Biochem. 1985 Nov 1;150(2):429–441. doi: 10.1016/0003-2697(85)90532-9. [DOI] [PubMed] [Google Scholar]
- Maniatis T., Hardison R. C., Lacy E., Lauer J., O'Connell C., Quon D., Sim G. K., Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. doi: 10.1016/0092-8674(78)90036-3. [DOI] [PubMed] [Google Scholar]
- McClanahan T., McEntee K. DNA damage and heat shock dually regulate genes in Saccharomyces cerevisiae. Mol Cell Biol. 1986 Jan;6(1):90–96. doi: 10.1128/mcb.6.1.90. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sarasin A. SOS response in mammalian cells. Cancer Invest. 1985;3(2):163–174. doi: 10.3109/07357908509017499. [DOI] [PubMed] [Google Scholar]
- Walker G. C. Inducible DNA repair systems. Annu Rev Biochem. 1985;54:425–457. doi: 10.1146/annurev.bi.54.070185.002233. [DOI] [PubMed] [Google Scholar]




