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. 1972 Jul;50(1):19–23. doi: 10.1104/pp.50.1.19

Protein Synthesis by Isolated Etioplasts and Chloroplasts from Pea and Wheat and the Effects of Chloramphenicol and Cycloheximide 1

Bonnie J Reger 1,2,2, R M Smillie 1,2,3, R C Fuller 1,2,4
PMCID: PMC367308  PMID: 16658121

Abstract

Etioplasts capable of incorporating 14C-leucine into protein have been isolated from dark-grown pea and wheat plants. The requirements for leucine incorporation for etioplasts were similar to those for chloroplasts. An ATP-generating system, Mg2+, and GTP were required. The amino-acid-incorporation activity of etioplasts from wheat was comparable to that of chloroplasts on an RNA basis, whereas the activity of pea etioplasts was about 50% of the activity of pea chloroplasts. The incorporation of leucine into protein by etioplasts and chloroplasts from pea and wheat was inhibited by chloramphenicol, and to a slight extent by cycloheximide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOARDMAN N. K., FRANCKI R. I., WILDMAN S. G. PROTEIN SYNTHESIS BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. II. ASSOCIATION OF ACTIVITY WITH CHLOROPLAST RIBOSOMES. Biochemistry. 1965 May;4:872–876. doi: 10.1021/bi00881a012. [DOI] [PubMed] [Google Scholar]
  2. BOLLUM F. J. Calf thymus polymerase. J Biol Chem. 1960 Aug;235:2399–2403. [PubMed] [Google Scholar]
  3. Bamji M. S., Jagendorf A. T. Amino Acid incorporation by wheat chloroplasts. Plant Physiol. 1966 May;41(5):764–770. doi: 10.1104/pp.41.5.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boardman N. K., Francki R. I., Wildman S. G. Protein synthesis by cell-free extracts of tobacco leaves. 3. Comparison of the physical properties and protein synthesizing activities of 70 s chloroplast and 80 s cytoplasmic ribosomes. J Mol Biol. 1966 Jun;17(2):470–487. doi: 10.1016/s0022-2836(66)80157-2. [DOI] [PubMed] [Google Scholar]
  5. Boardman N. K. Ribosome composition and chloroplast development in Phaseolus vulgaris. Exp Cell Res. 1966 Sep;43(2):474–482. doi: 10.1016/0014-4827(66)90074-7. [DOI] [PubMed] [Google Scholar]
  6. DAS H. K., CHATTERJEE S. K., ROY S. C. PROTEIN SYNTHESIS IN PLANT MITOCHONDRIA. II. GLUTAMATE AND GLUTAMINE INCORPORATION AND A STUDY OF INITIAL STEPS AND STREPTOMYCIN EFFECT. Biochim Biophys Acta. 1964 Jul 22;87:478–489. doi: 10.1016/0926-6550(64)90119-7. [DOI] [PubMed] [Google Scholar]
  7. Drumm H. E., Margulies M. M. In vitro protein synthesis by plastids of Phaseolus vulgaris. IV. Amino acid incorporation by etioplasts and effect of illumination of leaves on incorporation by plastids. Plant Physiol. 1970 Apr;45(4):435–442. doi: 10.1104/pp.45.4.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EISENSTADT J. M., BRAWERMAN G. THE PROTEIN-SYNTHESIZING SYSTEMS FROM THE CYTOPLASM AND THE CHLOROPLASTS OF EUGLENA GRACILIS. J Mol Biol. 1964 Dec;10:392–402. doi: 10.1016/s0022-2836(64)80060-7. [DOI] [PubMed] [Google Scholar]
  9. ENNIS H. L., LUBIN M. CYCLOHEXIMIDE: ASPECTS OF INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS. Science. 1964 Dec 11;146(3650):1474–1476. doi: 10.1126/science.146.3650.1474. [DOI] [PubMed] [Google Scholar]
  10. Ellis R. J. [Chloroplast ribosomes: stereospecificity of inhibition by chloramphenicol]. Science. 1969 Jan 31;163(3866):477–478. doi: 10.1126/science.163.3866.477. [DOI] [PubMed] [Google Scholar]
  11. FRANCKI R. I., BOARDMAN N. K., WILDMAN S. G. PROTEIN SYNTHESIS BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. I. AMINO ACID INCORPORATING ACTIVITY OF CHLOROPLASTS IN RELATION TO THEIR STRUCTURE. Biochemistry. 1965 May;4:865–872. doi: 10.1021/bi00881a011. [DOI] [PubMed] [Google Scholar]
  12. GOFFEAU A., BRACHET J. DEOXYRIBONUCLEIC ACID-DEPENDENT INCORPORATION OF AMINO ACIDS INTO THE PROTEINS OF CHLOROPLASTS ISOLATED FROM ANUCLEATE ACETABULARIA FRAGMENTS. Biochim Biophys Acta. 1965 Feb 8;95:302–313. doi: 10.1016/0005-2787(65)90494-6. [DOI] [PubMed] [Google Scholar]
  13. GUNNING B. E. THE FINE STRUCTURE OF CHLOROPLAST STROMA FOLLOWING ALDEHYDE OSMIUM-TETROXIDE FIXATION. J Cell Biol. 1965 Jan;24:79–93. doi: 10.1083/jcb.24.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gyldenholm A. O. Macromolecular physiology of plastids V. On the nucleic acid metabolism during chloroplast development. Hereditas. 1968;59(1):142–168. doi: 10.1111/j.1601-5223.1968.tb02168.x. [DOI] [PubMed] [Google Scholar]
  15. Hall T. C., Cocking E. C. Amino acid incorporation into protein by aseptic cell-free systems from tomato cotyledons and leaves. Biochim Biophys Acta. 1966 Jul 20;123(1):163–171. doi: 10.1016/0005-2787(66)90169-9. [DOI] [PubMed] [Google Scholar]
  16. Jacobson A. B. A procedure for isolation of proplastids from etiolated maize leaves. J Cell Biol. 1968 Jul;38(1):238–244. doi: 10.1083/jcb.38.1.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parenti F., Margulies M. M. In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing Leaves. Plant Physiol. 1967 Sep;42(9):1179–1186. doi: 10.1104/pp.42.9.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Parthier B. Chloramphenicol-Wirkung auf eine durch Licht stimulierte Proteinsynthesis in Blättern und in isolierten Chloroplasten. Z Naturforsch B. 1965 Dec;20(12):1191–1197. [PubMed] [Google Scholar]
  19. Rawson J. R., Stutz E. Isolation and characterization of Euglena gracilis cytoplasmic and chloroplast ribosomes and their ribosomal RNA components. Biochim Biophys Acta. 1969 Oct 22;190(2):368–380. doi: 10.1016/0005-2787(69)90087-2. [DOI] [PubMed] [Google Scholar]
  20. Reger B. J., Fairfield S. A., Epler J. L., Barnett W. E. Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1207–1213. doi: 10.1073/pnas.67.3.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reger B. J., Smillie R. M., Fuller R. C. Light-stimulated Production of a Chloroplast-localized System for Protein Synthesis in Euglena gracilis. Plant Physiol. 1972 Jul;50(1):24–27. doi: 10.1104/pp.50.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SPENCER D., WILDMAN S. G. THE INCORPORATION OF AMINO ACIDS INTO PROTEIN BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. Biochemistry. 1964 Jul;3:954–959. doi: 10.1021/bi00895a019. [DOI] [PubMed] [Google Scholar]
  23. Smillie R. M., Graham D., Dwyer M. R., Grieve A., Tobin N. F. Evidence for the synthesis in vivo of proteins of the Calvin cycle and of the photosynthetic electron-transfer pathway on chloroplast ribosomes. Biochem Biophys Res Commun. 1967 Aug 23;28(4):604–610. doi: 10.1016/0006-291x(67)90356-7. [DOI] [PubMed] [Google Scholar]
  24. Spencer D. Protein synthesis by isolated spinach chloroplasts. Arch Biochem Biophys. 1965 Aug;111(2):381–390. doi: 10.1016/0003-9861(65)90200-6. [DOI] [PubMed] [Google Scholar]

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