Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1972 Jul;50(1):31–34. doi: 10.1104/pp.50.1.31

Lipid Composition of Pea and Bean Leaves during Chloroplast Development

P G Roughan a,1, N K Boardman a
PMCID: PMC367311  PMID: 16658128

Abstract

The changes in composition of the complex lipids were followed during the greening of dark-grown pea (Pisum sativum) and bean (Phaseolus vulgaris) seedlings. No significant changes in glycerolipid concentrations in the leaves were observed during the early stages of greening (0-8 hour for peas and 0-12 hour for beans). On further greening, there was an increase in the proportion of galactolipids and a decrease in the phospholipids. The fatty acid composition of the galactolipids remained constant during 24 hours of greening, but there was a slight increase in α-linolenic acid at 72 hours in the bean. The percentage of α-linolenic acid in the phospholipids and in sulfolipid showed a marked increase between 24 and 72 hours in the bean. Trans-δ3-hexadecenoic acid was the major fatty acid of phosphatidyl glycerol in bean leaves at 72 hours, but it was barely detectable at 24 hours. The lipid composition of greening leaves is discussed in relation to the fine structure and photochemical activity of the developing plastids.

Full text

PDF
31

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ERWIN J., BLOCH K. POLYUNSATURATED FATTY ACIDS IN SOME PHOTOSYNTHETIC MICROORGANISMS. Biochem Z. 1963;338:496–511. [PubMed] [Google Scholar]
  2. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  3. McCarty R. E., Jagendorf A. T. Chloroplast damage due to enzymatic hydrolysis of endogenous lipids. Plant Physiol. 1965 Jul;40(4):725–735. doi: 10.1104/pp.40.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Morris L. J. Separations of lipids by silver ion chromatography. J Lipid Res. 1966 Nov;7(6):717–732. [PubMed] [Google Scholar]
  5. Nichols B. W. Light induced changes in the lipids of Chlorella vulgaris. Biochim Biophys Acta. 1965 Oct 4;106(2):274–279. doi: 10.1016/0005-2760(65)90035-4. [DOI] [PubMed] [Google Scholar]
  6. Rosenberg A., Gouaux J., Milch P. Monogalactosyl and digalactosyl diglycerides from heterotrophic, hetero-autotrophic, and photobiotic Euglena gracilis. J Lipid Res. 1966 Nov;7(6):733–738. [PubMed] [Google Scholar]
  7. Roughan P. G., Batt R. D. Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues. Anal Biochem. 1968 Jan;22(1):74–88. doi: 10.1016/0003-2697(68)90261-3. [DOI] [PubMed] [Google Scholar]
  8. Roughan P. G. Turnover of the glycerolipids of pumpkin leaves. The importence of phosphatidylcholine. Biochem J. 1970 Mar;117(1):1–8. doi: 10.1042/bj1170001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SASTRY P. S., KATES M. HYDROLYSIS OF MONOGALACTOSYL AND DIGALACTOSYL DIGLYCERIDES BY SPECIFIC ENZYMES IN RUNNER-BEAN LEAVES. Biochemistry. 1964 Sep;3:1280–1287. doi: 10.1021/bi00897a016. [DOI] [PubMed] [Google Scholar]
  10. Thomas T. D., Batt R. D. Synthesis of protein and ribonucleic acid by starved Streptococcus lactis in relation to survival. J Gen Microbiol. 1969 Nov;58(3):363–369. doi: 10.1099/00221287-58-3-363. [DOI] [PubMed] [Google Scholar]
  11. Thorne S. W., Boardman N. K. Formation of chlorophyll B, and the fluorescence properties and photochemical activities of isolated plastids from greening pea seedlings. Plant Physiol. 1971 Feb;47(2):252–261. doi: 10.1104/pp.47.2.252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Trémolières A., Lepage M. Changes in Lipid Composition during Greening of Etiolated Pea Seedlings. Plant Physiol. 1971 Feb;47(2):329–334. doi: 10.1104/pp.47.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wood B. J., Nichols B. W., James A. T. The lipids and fatty acid metabolism of photosynthetic bacteria. Biochim Biophys Acta. 1965 Oct 4;106(2):261–273. doi: 10.1016/0005-2760(65)90034-2. [DOI] [PubMed] [Google Scholar]
  14. Yang S. F., Freer S., Benson A. A. Transphosphatidylation by phospholipase D. J Biol Chem. 1967 Feb 10;242(3):477–484. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES