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. 1972 Jul;50(1):51–54. doi: 10.1104/pp.50.1.51

Intracellular Distribution of Mitochondria after Geotropic Stimulation of the Oat Coleoptile

J Shen-Miller a, Carol Miller a
PMCID: PMC367314  PMID: 16658131

Abstract

The number of mitochondria is greater in the bottom than in the top of cells in geotropically stimulated oat (Avena sativa) coleoptiles. In the avascular tip and outer epidermis of subapical regions this difference occurs only in the lower tissues. These inequalities are found both in the KMnO4− and in the glutaraldehyde-fixed tissues; however, they are significant only in the former. Also, the number of mitochondria scored is consistently lower when the tissues were fixed in KMnO4. These results suggest that mitochondria undergo a small degree of sedimentation after geostimulation, a redistribution reduced by the slower fixation with glutaraldehyde. Differences in mitochondrial number begin later than those in the amyloplast and the Golgi apparatus after geotropic stimulation. The cells in the avascular-tip region (a region having an important role in geotropism) have two to three times more mitochondria than the subapical cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burstein C., Kandrach A., Racker E. Effect of phospholipases and lipase on submitochondrial particles. J Biol Chem. 1971 Jun 25;246(12):4083–4089. [PubMed] [Google Scholar]
  2. Burstein C., Loyter A., Racker E. Effect of phospholipases on the structure and function of mitochondria. J Biol Chem. 1971 Jun 25;246(12):4075–4082. [PubMed] [Google Scholar]
  3. Calvayrac R., Van Lente F., Butow R. A. Euglena gracilis: formation of giant mitochondria. Science. 1971 Jul 16;173(3993):252–254. doi: 10.1126/science.173.3993.252. [DOI] [PubMed] [Google Scholar]
  4. Criddle R. S., Schatz G. Promitochondria of anaerobically grown yeast. I. Isolation and biochemical properties. Biochemistry. 1969 Jan;8(1):322–334. doi: 10.1021/bi00829a045. [DOI] [PubMed] [Google Scholar]
  5. Gambetti P., Gonatas N. K., Flexner L. B. Puromycin: action on neuronal mitochondria. Science. 1968 Aug 30;161(3844):900–902. doi: 10.1126/science.161.3844.900. [DOI] [PubMed] [Google Scholar]
  6. Miller R. J., Bell D. T., Koeppe D. E. The effects of water stress on some membrane characteristics of corn mitochondria. Plant Physiol. 1971 Aug;48(2):229–231. doi: 10.1104/pp.48.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pickard B. G., Thimann K. V. Geotropic response of wheat coleoptiles in absence of amyloplast starch. J Gen Physiol. 1966 May;49(5):1065–1086. doi: 10.1085/jgp.49.5.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shen-Miller J., Miller C. Distribution and activation of the Golgi apparatus in geotropism. Plant Physiol. 1972 Apr;49(4):634–639. doi: 10.1104/pp.49.4.634. [DOI] [PMC free article] [PubMed] [Google Scholar]

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