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. 1973 Jan;51(1):22–25. doi: 10.1104/pp.51.1.22

Changes in Certain Aminoacyl Transfer Ribonucleic Acid Synthetase Activities in Developing Pea Roots 1

Joe R Cowles a,2, Joe L Key a
PMCID: PMC367350  PMID: 16658289

Abstract

Tyrosyl-, arginyl-, leucyl-, and phenylalanyl-tRNA synthetase activities were measured in extracts from three root sections of 3-day-old pea seedlings. The sections 0 to 2, 3 to 7, and 8 to 22 millimeters from the root tip were chosen to represent the regions of cell division, elongation, and maturation, respectively. The specific activity for each aminoacyl-tRNA synthetase was highest in the 0- to 2-millimeter section and lowest in the 8 to 22 millimeter section. The changes in specific activity between the sections, however, varied with the particular synthetase. Tyrosyl-tRNA synthetase from each section was fractionated into two activity regions on a diethylaminoethyl cellulose column. Approximately 10, 22, and 44% of the total tyrosyl-tRNA synthetase activity in the 0 to 2, 3 to 7, and 8- to 22-millimeter sections, respectively, were associated with the first tyrosyl-tRNA synthetase region; the remaining activity was located in the second tyrosyl-tRNA synthetase region. Only one activity region for arginyl-tRNA synthetase was detected by diethylaminoethyl cellulose column fractionation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. B., Cherry J. H. Differences in leucyl-transfer rna's and synthetase in soybean seedlings. Proc Natl Acad Sci U S A. 1969 Jan;62(1):202–209. doi: 10.1073/pnas.62.1.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Axel R., Weinstein B., Farber E. Patterns of transfer RNA in normal rat liver and during hepatic carcinogenesis. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1255–1260. doi: 10.1073/pnas.58.3.1255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bick M. D., Liebke H., Cherry J. H., Strehler B. L. Changes in leucyl- and tyrosyl-tRNA of soybean cotyledons during plant growth. Biochim Biophys Acta. 1970 Mar 19;204(1):175–182. doi: 10.1016/0005-2787(70)90500-9. [DOI] [PubMed] [Google Scholar]
  4. Cowles J. R., Key J. L. Demonstration of two tyrosyl-tRNA synthetases of pea roots. Biochim Biophys Acta. 1972 Sep 29;281(1):33–44. doi: 10.1016/0005-2787(72)90185-2. [DOI] [PubMed] [Google Scholar]
  5. Kanabus J., Cherry J. H. Isolation of an organ-specific leucyl-tRNA synthetase from soybean seedling. Proc Natl Acad Sci U S A. 1971 May;68(5):873–876. doi: 10.1073/pnas.68.5.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaneko I., Doi R. H. Alteration of valyl-sRNA during sporulation of bacillus subtilis. Proc Natl Acad Sci U S A. 1966 Mar;55(3):564–571. doi: 10.1073/pnas.55.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lazzarini R. A. Differences in lysine-sRNA from spore and vegetative cells of Bacillus subtillis. Proc Natl Acad Sci U S A. 1966 Jul;56(1):185–190. doi: 10.1073/pnas.56.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee J. C., Ingram V. M. Erythrocyte transfer RNA: change during chick development. Science. 1967 Dec 8;158(3806):1330–1332. doi: 10.1126/science.158.3806.1330. [DOI] [PubMed] [Google Scholar]
  9. Vanderhoef L. N., Key J. L. The fractionation of transfer ribonucleic Acid from roots of pea seedlings. Plant Physiol. 1970 Aug;46(2):294–298. doi: 10.1104/pp.46.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vold B. S., Sypherd P. S. Modification in transfer RNA during the differentiation of wheat seedlings. Proc Natl Acad Sci U S A. 1968 Feb;59(2):453–458. doi: 10.1073/pnas.59.2.453. [DOI] [PMC free article] [PubMed] [Google Scholar]

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