Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1973 Jan;51(1):82–88. doi: 10.1104/pp.51.1.82

Action Spectra for Guard Cell Rb+ Uptake and Stomatal Opening in Vivia faba

Theodore C Hsiao a,1, W G Allaway a, L T Evans b
PMCID: PMC367361  PMID: 16658302

Abstract

Abaxial epidermal strips, containing guard cells as the only viable cells, were prepared from leaves of Vicia faba following a period in darkness, and floated, under CO2-free air, on 2 mm RbCl + 0.1 mm CaCl2 labeled with 86Rb+. Under white light (high pressure mercury vapor lamp), stomatal opening in these strips approached its maximum at less than 0.02 calorie per square centimeter per minute. Under light of different wavelengths, 20 nanometers apart, and at a low quantum flux density of 7 × 1014 quanta per square centimeter per second, Rb+ uptake and stomatal opening were activated only in the blue and long ultraviolet regions, with a peak at 420 to 460 nanometers. The action spectrum suggests that the underlying process is not photosynthesis. At higher quantum flux density (38 × 1014 quanta per square centimeter per second), uptake and opening also responded to red (600-680 nanometers) and somewhat to green light, with a minimum at 540 to 560 nanometers, indicating a possible involvement of the photosynthetic process. This light-induced opening appeared not to be mediated by a lowering of CO2 concentration, since CO2-free air was used in all treatments and controls. Stomatal opening paralleled Rb+ uptake in all cases. This constitutes further evidence for the potassium transport hypothesis of stomatal movement.

In the abaxial surface of leaf discs under air of normal CO2 concentration, stomatal opening in white light approached its maximum at an intensity similar to that for epidermal strips. At both quantum flux densities, the action spectra for opening in leaf discs were very similar to those for epidermal strips. Thus, these light-linked processes for stomatal opening are likely to be the same in leaves as in epidermal strips.

Full text

PDF
82

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fischer R. A., Hsiao T. C. Stomatal Opening in Isolated Epidermal Strips of Vicia faba. II. Responses to KCl Concentration and the Role of Potassium Absorption. Plant Physiol. 1968 Dec;43(12):1953–1958. doi: 10.1104/pp.43.12.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fischer R. A. Stomatal Opening in Isolated Epidermal Strips of Vicia faba. I. Response to Light and to CO(2)-free Air. Plant Physiol. 1968 Dec;43(12):1947–1952. doi: 10.1104/pp.43.12.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fischer R. A. Stomatal opening: role of potassium uptake by guard cells. Science. 1968 May 17;160(3829):784–785. doi: 10.1126/science.160.3829.784. [DOI] [PubMed] [Google Scholar]
  4. Halle W., Wollenberger A. Die Erhaltung der Automatie von Herzzellen in einem chemisch definierten Nährmedium in vitro. Naturwissenschaften. 1967 Feb;54(3):72–72. [PubMed] [Google Scholar]
  5. Humble G. D., Hsiao T. C. Light-dependent Influx and Efflux of Potassium of Guard Cells during Stomatal Opening and Closing. Plant Physiol. 1970 Sep;46(3):483–487. doi: 10.1104/pp.46.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Humble G. D., Hsiao T. C. Specific requirement of potassium for light-activated opening of stomata in epidermal strips. Plant Physiol. 1969 Feb;44(2):230–234. doi: 10.1104/pp.44.2.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Humble G. D., Raschke K. Stomatal opening quantitatively related to potassium transport: evidence from electron probe analysis. Plant Physiol. 1971 Oct;48(4):447–453. doi: 10.1104/pp.48.4.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kowallik W. Action spectrum for an enhancement of endogenous respiration by light in chlorella. Plant Physiol. 1967 May;42(5):672–676. doi: 10.1104/pp.42.5.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kuiper P. J. Dependence upon Wavelength of Stomatal Movement in Epidermal Tissue of Senecio odoris. Plant Physiol. 1964 Nov;39(6):952–955. doi: 10.1104/pp.39.6.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Läuchli A., Epstein E. Transport of potassium and rubidium in plant roots: the significance of calcium. Plant Physiol. 1970 May;45(5):639–641. doi: 10.1104/pp.45.5.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rains D. W. Kinetics and Energetics of Light-enhanced Potassium Absorption by Corn Leaf Tissue. Plant Physiol. 1968 Mar;43(3):394–400. doi: 10.1104/pp.43.3.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sawhney B. L., Zelitch I. Direct determination of potassium ion accumulation in guard cells in relation to stomatal opening in light. Plant Physiol. 1969 Sep;44(9):1350–1354. doi: 10.1104/pp.44.9.1350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES